Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle

Yong Taik Lim, Kun Yeong Lee, Kwangyeol Lee, Bong Hyun Chung

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We described the development of functionalized magnetic nanoparticles (MNPs) with PEG-modification, a phospholipids micelle coating, and their use in manipulating histidine-tagged proteins. Highly monodisperse MNPs were synthesized in an organic solvent and could be phase-transferred into an aqueous solution by encapsulating the nanoparticles with a phospholipids micelle. The phospholipids micelle coating rendered the nanoparticles highly water-soluble, and the functional groups of the phospholipids coating allowed for the bioconjugation of various moieties, such as fluorescent molecules and engineered proteins. Functionalized phospholipids, such as nitrilotriacetic acid (NTA)-phospholipids, caused the MNPs to bind and allowed for manipulation of histidine-tagged proteins. Due to their high surface/volume ratio, the MNPs showed better performance (about 100 times higher) in immobilizing engineered proteins than conventional micrometer-sized beads. This demonstrates that MNPs coated with phospholipids micelle can be a versatile platform for the effective manipulation of various kinds of engineered proteins, which is very important in the field of proteomics. It is expected that a combination of MNPs with optical fluorescent molecules can find applications in bimodal (magnetic and optical) molecular imaging nanoprobes.

Original languageEnglish
Pages (from-to)926-930
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume344
Issue number3
DOIs
Publication statusPublished - 2006 Jun 9

Fingerprint

Nitrilotriacetic Acid
Micelles
Histidine
Immobilization
Nanoparticles
Phospholipids
Proteins
Coatings
Nanoprobes
Molecular imaging
Molecules
Molecular Imaging
Optical Imaging
Proteomics
Organic solvents
Functional groups
Polyethylene glycols

Keywords

  • Fluorescent proteins
  • Immobilization of proteins
  • Magnetic nanoparticles
  • NTA-phospholipids
  • Phospholipids micelle

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle. / Lim, Yong Taik; Lee, Kun Yeong; Lee, Kwangyeol; Chung, Bong Hyun.

In: Biochemical and Biophysical Research Communications, Vol. 344, No. 3, 09.06.2006, p. 926-930.

Research output: Contribution to journalArticle

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