Immobilization of lipase on single walled carbon nanotubes in ionic liquid

Han Ki Lee, Jae Kwan Lee, Mahn Joo Kim, Cheol Jin Lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-BF4). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.

Original languageEnglish
Pages (from-to)650-652
Number of pages3
JournalBulletin of the Korean Chemical Society
Volume31
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

Fingerprint

Ionic Liquids
Single-walled carbon nanotubes (SWCN)
Lipase
Phenylethyl Alcohol
Immobilized Enzymes
Carbon Nanotubes
Transesterification
Toluene
Buffers
Esters
1-butyl-3-methylimidazolium tetrafluoroborate

Keywords

  • Biocatalysis
  • Immobilization
  • Ionic liquid
  • Lipase
  • Single walled carbon nanotube

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Immobilization of lipase on single walled carbon nanotubes in ionic liquid. / Lee, Han Ki; Lee, Jae Kwan; Kim, Mahn Joo; Lee, Cheol Jin.

In: Bulletin of the Korean Chemical Society, Vol. 31, No. 3, 01.03.2010, p. 650-652.

Research output: Contribution to journalArticle

Lee, Han Ki ; Lee, Jae Kwan ; Kim, Mahn Joo ; Lee, Cheol Jin. / Immobilization of lipase on single walled carbon nanotubes in ionic liquid. In: Bulletin of the Korean Chemical Society. 2010 ; Vol. 31, No. 3. pp. 650-652.
@article{194b6b26f92f4f4695613d8fb0f354e4,
title = "Immobilization of lipase on single walled carbon nanotubes in ionic liquid",
abstract = "A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-BF4). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.",
keywords = "Biocatalysis, Immobilization, Ionic liquid, Lipase, Single walled carbon nanotube",
author = "Lee, {Han Ki} and Lee, {Jae Kwan} and Kim, {Mahn Joo} and Lee, {Cheol Jin}",
year = "2010",
month = "3",
day = "1",
doi = "10.5012/bkcs.2010.31.03.650",
language = "English",
volume = "31",
pages = "650--652",
journal = "Bulletin of the Korean Chemical Society",
issn = "0253-2964",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Immobilization of lipase on single walled carbon nanotubes in ionic liquid

AU - Lee, Han Ki

AU - Lee, Jae Kwan

AU - Kim, Mahn Joo

AU - Lee, Cheol Jin

PY - 2010/3/1

Y1 - 2010/3/1

N2 - A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-BF4). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.

AB - A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-BF4). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.

KW - Biocatalysis

KW - Immobilization

KW - Ionic liquid

KW - Lipase

KW - Single walled carbon nanotube

UR - http://www.scopus.com/inward/record.url?scp=77951819763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951819763&partnerID=8YFLogxK

U2 - 10.5012/bkcs.2010.31.03.650

DO - 10.5012/bkcs.2010.31.03.650

M3 - Article

AN - SCOPUS:77951819763

VL - 31

SP - 650

EP - 652

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

SN - 0253-2964

IS - 3

ER -