Immobilization of Penicillium citrinum by entrapping cells in calcium alginate for the production of neo-fructooligosaccharides

Jung Soo Lim, Seung Won Park, Jin Won Lee, Kyeong Keon Oh, Seung Wook Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This work describes neo-fructooligosaccharides (neo-FOSs) production using the immobilized mycelia of Penicillium citrinum. Some critical factors were evaluated to optimize maximal production of neo-FOS. Optimal alginate and cell concentrations were determined to be 1.96% alginate and 7.17% cell, respectively, by statistical analysis. The optimal concentration of CaCl2, which is related to bead stability, was determined to be 2 M. It was possible to increase the neo-FOS production by adding 15 units of glucose oxidase to the batch reaction. By co-immobilizing cells and glucose oxidase, neo-FOS productivity increased 123% compared with the whole-cell immobilization process. Based on the results above, a co-immobilization technique was developed and it can be utilized for large-scale production.

Original languageEnglish
Pages (from-to)1317-1322
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume15
Issue number6
Publication statusPublished - 2005 Dec 1

Fingerprint

Penicillium
Alginate
Immobilization
Calcium
Glucose Oxidase
Glucose oxidase
Cell immobilization
Mycelium
Statistical methods
Productivity
alginic acid
fructooligosaccharide

Keywords

  • Calcium alginate immobilization
  • Co-immobilization
  • Neo-fructooligosaccharide
  • Penicillium citrinum

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Immobilization of Penicillium citrinum by entrapping cells in calcium alginate for the production of neo-fructooligosaccharides. / Lim, Jung Soo; Park, Seung Won; Lee, Jin Won; Oh, Kyeong Keon; Kim, Seung Wook.

In: Journal of Microbiology and Biotechnology, Vol. 15, No. 6, 01.12.2005, p. 1317-1322.

Research output: Contribution to journalArticle

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AU - Oh, Kyeong Keon

AU - Kim, Seung Wook

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