Immobilization of Penicillium citrinum by entrapping cells in calcium alginate for the production of neo-fructooligosaccharides

Jung Soo Lim, Seung Won Park, Jin Won Lee, Kyeong Keon Oh, Seung Wook Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This work describes neo-fructooligosaccharides (neo-FOSs) production using the immobilized mycelia of Penicillium citrinum. Some critical factors were evaluated to optimize maximal production of neo-FOS. Optimal alginate and cell concentrations were determined to be 1.96% alginate and 7.17% cell, respectively, by statistical analysis. The optimal concentration of CaCl2, which is related to bead stability, was determined to be 2 M. It was possible to increase the neo-FOS production by adding 15 units of glucose oxidase to the batch reaction. By co-immobilizing cells and glucose oxidase, neo-FOS productivity increased 123% compared with the whole-cell immobilization process. Based on the results above, a co-immobilization technique was developed and it can be utilized for large-scale production.

Original languageEnglish
Pages (from-to)1317-1322
Number of pages6
JournalJournal of microbiology and biotechnology
Volume15
Issue number6
Publication statusPublished - 2005 Dec

Keywords

  • Calcium alginate immobilization
  • Co-immobilization
  • Neo-fructooligosaccharide
  • Penicillium citrinum

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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