Immobilization of Pholiota adiposa xylanase onto SiO 2 nanoparticles and its application for production of xylooligosaccharides

Saurabh Sudha Dhiman, Sujit Sadashiv Jagtap, Marimuthu Jeya, Jung Rim Haw, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Enhanced yields of different lignocellulases were obtained under statistically-optimized parameters using Pholiota adiposa. The k cat value (4,261 s -1) of purified xylanase under standard assay conditions was the highest value ever reported. On covalent immobilization of the crude xylanase preparation onto functionalized silicon oxide nanoparticles, 66 % of the loaded enzyme was retained on the particle. Immobilized enzyme gave 45 % higher concentrations of xylooligosaccharides compared to the free enzyme. After 17 cycles, the immobilized enzyme retained 97 % of the original activity, demonstrating its prospects for the synthesis of xylooligosaccharides in industrial applications.

Original languageEnglish
Pages (from-to)1307-1313
Number of pages7
JournalBiotechnology letters
Volume34
Issue number7
DOIs
Publication statusPublished - 2012 Jul 1
Externally publishedYes

Fingerprint

Pholiota
Immobilized Enzymes
Immobilization
Nanoparticles
Enzymes
Silicon oxides
Silicon
Oxides
Industrial applications
Assays
Cats
xylooligosaccharide

Keywords

  • Immobilization
  • Response surface methodology
  • SiO
  • Xylanase
  • Xylooligosaccharide

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Immobilization of Pholiota adiposa xylanase onto SiO 2 nanoparticles and its application for production of xylooligosaccharides. / Dhiman, Saurabh Sudha; Jagtap, Sujit Sadashiv; Jeya, Marimuthu; Haw, Jung Rim; Kang, Yun Chan; Lee, Jung Kul.

In: Biotechnology letters, Vol. 34, No. 7, 01.07.2012, p. 1307-1313.

Research output: Contribution to journalArticle

Dhiman, Saurabh Sudha ; Jagtap, Sujit Sadashiv ; Jeya, Marimuthu ; Haw, Jung Rim ; Kang, Yun Chan ; Lee, Jung Kul. / Immobilization of Pholiota adiposa xylanase onto SiO 2 nanoparticles and its application for production of xylooligosaccharides. In: Biotechnology letters. 2012 ; Vol. 34, No. 7. pp. 1307-1313.
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