Immobilization of Pholiota adiposa xylanase onto SiO 2 nanoparticles and its application for production of xylooligosaccharides

Saurabh Sudha Dhiman, Sujit Sadashiv Jagtap, Marimuthu Jeya, Jung Rim Haw, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Enhanced yields of different lignocellulases were obtained under statistically-optimized parameters using Pholiota adiposa. The k cat value (4,261 s -1) of purified xylanase under standard assay conditions was the highest value ever reported. On covalent immobilization of the crude xylanase preparation onto functionalized silicon oxide nanoparticles, 66 % of the loaded enzyme was retained on the particle. Immobilized enzyme gave 45 % higher concentrations of xylooligosaccharides compared to the free enzyme. After 17 cycles, the immobilized enzyme retained 97 % of the original activity, demonstrating its prospects for the synthesis of xylooligosaccharides in industrial applications.

Original languageEnglish
Pages (from-to)1307-1313
Number of pages7
JournalBiotechnology letters
Volume34
Issue number7
DOIs
Publication statusPublished - 2012 Jul
Externally publishedYes

Keywords

  • Immobilization
  • Response surface methodology
  • SiO
  • Xylanase
  • Xylooligosaccharide

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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