Immobilized cell microchannel bioreactor for evaluating fermentation characteristics of mixed substrate consumption and product formation

Tae Young Seo, Ki Won Eum, Sung Ok Han, Seung Wook Kim, Ji Hyeon Kim, Kwang Ho Song, Jaehoon Choe

Research output: Contribution to journalArticle

8 Citations (Scopus)


An immobilized cell microchannel bioreactor was designed to test continuous fermentation. The fermentation set-up included a bottom hydrophilic quartz channel to immobilize cells using 0.4 wt% polyethyleneimine and a top channel designed to continuously remove metabolically generated carbon dioxide using hydrophobic polypropylene. To evaluate fermentation characteristics of immobilized cells, ethanol fermentation was carried out using Saccharomyces cerevisiae and Pichia stipitis. The immobilized cell microchannel bioreactor was used to identify long-term activity of immobilized S. cerevisiae cells. The continuous flow microchannel bioreactor was operated stably over a period of 1 month. The immobilized cell microchannel bioreactor was used to examine the characteristics cells that consumed mixed substrates. The concentration ratio of glucose to xylose for simultaneous utilization of hemicellulosic sugars was evaluated using the microchannel bioreactor and the results were compared with those obtained by using conventional batch fermentation with P. stipitis.

Original languageEnglish
Pages (from-to)1011-1015
Number of pages5
JournalProcess Biochemistry
Issue number6
Publication statusPublished - 2012 Jun 1



  • Catabolite repression
  • Continuous fermentation
  • Immobilized cell microchannel bioreactor
  • Pichia stipitis
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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