Immobilized Phospholipase A1-Catalyzed Preparation of l -α-Glycerylphosphorylcholine from Phosphatidylcholine

Yejin Song, Seoye Roh, Jihyun Hwang, Min Yu Chung, In Hwan Kim, Byung Hee Kim

Research output: Contribution to journalArticlepeer-review

Abstract

This study sought to prepare a cognitive enhancer l-α-glycerylphosphorylcholine (l-α-GPC) using an immobilized Lecitase Ultra (LU, phospholipase A1) to catalyze the hydrolysis of soy phosphatidylcholine (PC). Immobilization of LU on Lewatit VP OC 1600 provided the highest fixation level (83.1 g/100 g) and greatest catalytic activity achieving 100 g/100 g l-α-GPC within 20 h and was therefore selected as the optimal system for biocatalysis. Immobilization of LU increased its positional specificity compared to free LU, as shown by a decrease in the production of the phosphocholine byproduct. Under the optimal conditions determined by response surface methodology, PC was completely hydrolyzed to l-α-GPC and required a simple purification via phase separation of the biphasic media to obtain a yield of ∼26.4 g l-α-GPC from 100 g PC, with a purity of 98.5 g/100 g. Our findings suggest a possibility of using the immobilized LU as a new biocatalyst for the l-α-GPC production.

Original languageEnglish
Pages (from-to)12375-12383
Number of pages9
JournalJournal of agricultural and food chemistry
Volume68
Issue number44
DOIs
Publication statusPublished - 2020 Nov 4

Keywords

  • cognitive enhancer
  • immobilization
  • interfacial activation
  • l -α-glycerylphosphorylcholine
  • phospholipase A

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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