Immune-enhancing alkali-soluble glucans produced by wild-type and mutant Saccharomyces cerevisiae

Chang Hoon Ha, Ki Hong Lim, Se Hwan Jang, Cheol Won Yun, Hyun Dong Paik, Seung Wook Kim, Chang Won Kang, Hyo Ihl Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The alkali-soluble glucan of the yeast cell wall contains β-(1,3)- and (1,6)-D-linkages and is known to systemically enhance the immune system. In the previous study [6], in order to isolate cell wall mutants, a wild-type strain was mutagenized by exposure to ultraviolet light, and the mutants were then selected via treatment with laminarinase (endo-β-(1,3)-D-glucanase). The mass of alkali- and water-soluble glucans produced by the mutant was measured to be 33.8 mg/g of the dry mass of the yeast cell. Our results showed that the mutants generated the amount of alkali-soluble glucan 10-fold higher than that generated by the wild-type. Structural analysis showed that the alkali-soluble glucan from the mutants was associated with a higher degree of β-(1,6)-D-linkage than was observed in conjunction with the wild-type. Yeast cell wall β-glucan was shown to interact with macrophages via receptors, thereby inducing the release of tumor necrosis factor alpha (TNF-α) and nitric oxide. Alkali-soluble β-glucans, both from water-soluble and water-insoluble glucan, exhibited a higher degree of macrophage activity with regard to both the secretion of tumor necrosis factor alpha (TNF-α) and nitric oxide and direct phagocytosis, than did the positive control (1 μg of lipopolysaccharide).

Original languageEnglish
Pages (from-to)576-583
Number of pages8
JournalJournal of microbiology and biotechnology
Volume16
Issue number4
Publication statusPublished - 2006 Apr

Keywords

  • Alkali-soluble glucan
  • Mannoprotein
  • Random mutation
  • Yeast cell wall

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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