Immune-enhancing alkali-soluble glucans produced by wild-type and mutant Saccharomyces cerevisiae

Chang Hoon Ha, Ki Hong Lim, Se Hwan Jang, Cheol-Won Yun, Hyun Dong Paik, Seung Wook Kim, Chang Won Kang, Hyo-Ihl Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The alkali-soluble glucan of the yeast cell wall contains β-(1,3)- and (1,6)-D-linkages and is known to systemically enhance the immune system. In the previous study [6], in order to isolate cell wall mutants, a wild-type strain was mutagenized by exposure to ultraviolet light, and the mutants were then selected via treatment with laminarinase (endo-β-(1,3)-D-glucanase). The mass of alkali- and water-soluble glucans produced by the mutant was measured to be 33.8 mg/g of the dry mass of the yeast cell. Our results showed that the mutants generated the amount of alkali-soluble glucan 10-fold higher than that generated by the wild-type. Structural analysis showed that the alkali-soluble glucan from the mutants was associated with a higher degree of β-(1,6)-D-linkage than was observed in conjunction with the wild-type. Yeast cell wall β-glucan was shown to interact with macrophages via receptors, thereby inducing the release of tumor necrosis factor alpha (TNF-α) and nitric oxide. Alkali-soluble β-glucans, both from water-soluble and water-insoluble glucan, exhibited a higher degree of macrophage activity with regard to both the secretion of tumor necrosis factor alpha (TNF-α) and nitric oxide and direct phagocytosis, than did the positive control (1 μg of lipopolysaccharide).

Original languageEnglish
Pages (from-to)576-583
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume16
Issue number4
Publication statusPublished - 2006 Apr 1

Fingerprint

Glucans
Alkalies
Yeast
Saccharomyces cerevisiae
Cells
Macrophages
Nitric oxide
Cell Wall
Yeasts
Water
Immune system
Structural analysis
Nitric Oxide
Tumor Necrosis Factor-alpha
Cellulases
Ultraviolet Rays
Phagocytosis
Lipopolysaccharides
Immune System

Keywords

  • Alkali-soluble glucan
  • Mannoprotein
  • Random mutation
  • Yeast cell wall

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Immune-enhancing alkali-soluble glucans produced by wild-type and mutant Saccharomyces cerevisiae. / Ha, Chang Hoon; Lim, Ki Hong; Jang, Se Hwan; Yun, Cheol-Won; Paik, Hyun Dong; Kim, Seung Wook; Kang, Chang Won; Chang, Hyo-Ihl.

In: Journal of Microbiology and Biotechnology, Vol. 16, No. 4, 01.04.2006, p. 576-583.

Research output: Contribution to journalArticle

Ha, Chang Hoon ; Lim, Ki Hong ; Jang, Se Hwan ; Yun, Cheol-Won ; Paik, Hyun Dong ; Kim, Seung Wook ; Kang, Chang Won ; Chang, Hyo-Ihl. / Immune-enhancing alkali-soluble glucans produced by wild-type and mutant Saccharomyces cerevisiae. In: Journal of Microbiology and Biotechnology. 2006 ; Vol. 16, No. 4. pp. 576-583.
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