Abstract
The alkali-soluble glucan of the yeast cell wall contains β-(1,3)- and (1,6)-D-linkages and is known to systemically enhance the immune system. In the previous study [6], in order to isolate cell wall mutants, a wild-type strain was mutagenized by exposure to ultraviolet light, and the mutants were then selected via treatment with laminarinase (endo-β-(1,3)-D-glucanase). The mass of alkali- and water-soluble glucans produced by the mutant was measured to be 33.8 mg/g of the dry mass of the yeast cell. Our results showed that the mutants generated the amount of alkali-soluble glucan 10-fold higher than that generated by the wild-type. Structural analysis showed that the alkali-soluble glucan from the mutants was associated with a higher degree of β-(1,6)-D-linkage than was observed in conjunction with the wild-type. Yeast cell wall β-glucan was shown to interact with macrophages via receptors, thereby inducing the release of tumor necrosis factor alpha (TNF-α) and nitric oxide. Alkali-soluble β-glucans, both from water-soluble and water-insoluble glucan, exhibited a higher degree of macrophage activity with regard to both the secretion of tumor necrosis factor alpha (TNF-α) and nitric oxide and direct phagocytosis, than did the positive control (1 μg of lipopolysaccharide).
| Original language | English |
|---|---|
| Pages (from-to) | 576-583 |
| Number of pages | 8 |
| Journal | Journal of Microbiology and Biotechnology |
| Volume | 16 |
| Issue number | 4 |
| Publication status | Published - 2006 Apr 1 |
Fingerprint
Keywords
- Alkali-soluble glucan
- Mannoprotein
- Random mutation
- Yeast cell wall
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Microbiology
- Bioengineering
Cite this
Immune-enhancing alkali-soluble glucans produced by wild-type and mutant Saccharomyces cerevisiae. / Ha, Chang Hoon; Lim, Ki Hong; Jang, Se Hwan; Yun, Cheol-Won; Paik, Hyun Dong; Kim, Seung Wook; Kang, Chang Won; Chang, Hyo-Ihl.
In: Journal of Microbiology and Biotechnology, Vol. 16, No. 4, 01.04.2006, p. 576-583.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immune-enhancing alkali-soluble glucans produced by wild-type and mutant Saccharomyces cerevisiae
AU - Ha, Chang Hoon
AU - Lim, Ki Hong
AU - Jang, Se Hwan
AU - Yun, Cheol-Won
AU - Paik, Hyun Dong
AU - Kim, Seung Wook
AU - Kang, Chang Won
AU - Chang, Hyo-Ihl
PY - 2006/4/1
Y1 - 2006/4/1
N2 - The alkali-soluble glucan of the yeast cell wall contains β-(1,3)- and (1,6)-D-linkages and is known to systemically enhance the immune system. In the previous study [6], in order to isolate cell wall mutants, a wild-type strain was mutagenized by exposure to ultraviolet light, and the mutants were then selected via treatment with laminarinase (endo-β-(1,3)-D-glucanase). The mass of alkali- and water-soluble glucans produced by the mutant was measured to be 33.8 mg/g of the dry mass of the yeast cell. Our results showed that the mutants generated the amount of alkali-soluble glucan 10-fold higher than that generated by the wild-type. Structural analysis showed that the alkali-soluble glucan from the mutants was associated with a higher degree of β-(1,6)-D-linkage than was observed in conjunction with the wild-type. Yeast cell wall β-glucan was shown to interact with macrophages via receptors, thereby inducing the release of tumor necrosis factor alpha (TNF-α) and nitric oxide. Alkali-soluble β-glucans, both from water-soluble and water-insoluble glucan, exhibited a higher degree of macrophage activity with regard to both the secretion of tumor necrosis factor alpha (TNF-α) and nitric oxide and direct phagocytosis, than did the positive control (1 μg of lipopolysaccharide).
AB - The alkali-soluble glucan of the yeast cell wall contains β-(1,3)- and (1,6)-D-linkages and is known to systemically enhance the immune system. In the previous study [6], in order to isolate cell wall mutants, a wild-type strain was mutagenized by exposure to ultraviolet light, and the mutants were then selected via treatment with laminarinase (endo-β-(1,3)-D-glucanase). The mass of alkali- and water-soluble glucans produced by the mutant was measured to be 33.8 mg/g of the dry mass of the yeast cell. Our results showed that the mutants generated the amount of alkali-soluble glucan 10-fold higher than that generated by the wild-type. Structural analysis showed that the alkali-soluble glucan from the mutants was associated with a higher degree of β-(1,6)-D-linkage than was observed in conjunction with the wild-type. Yeast cell wall β-glucan was shown to interact with macrophages via receptors, thereby inducing the release of tumor necrosis factor alpha (TNF-α) and nitric oxide. Alkali-soluble β-glucans, both from water-soluble and water-insoluble glucan, exhibited a higher degree of macrophage activity with regard to both the secretion of tumor necrosis factor alpha (TNF-α) and nitric oxide and direct phagocytosis, than did the positive control (1 μg of lipopolysaccharide).
KW - Alkali-soluble glucan
KW - Mannoprotein
KW - Random mutation
KW - Yeast cell wall
UR - http://www.scopus.com/inward/record.url?scp=33646572203&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646572203&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33646572203
VL - 16
SP - 576
EP - 583
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
SN - 1017-7825
IS - 4
ER -