Immunogenicity and antigenicity of Plasmodium vivax merozoite surface protein 10

Yang Cheng, Bo Wang, Jetsumon Sattabongkot, Chae Seung Lim, Takafumi Tsuboi, Eun Taek Han

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Among the proteins involved in the invasion by merozoite, the glycosylphosphatidylinositol-anchored proteins (GPI-APs) are suggested as potential vaccine candidates because of their localization to apical organelles and the surface; these candidates are predicted to play essential roles during invasion. As a GPI-AP, Plasmodium vivax merozoite surface protein 10 (PvMSP-10) induces high antibody titers. However, such high antibody titers have shown no protective efficacy for animals challenged with P. vivax parasites in a previous study. To adequately evaluate the immunogenicity and further characterize PvMSP-10 in order to understand its vaccine potential, we assessed its immunogenicity by immunizing BALB/c mice with cell-free expressed recombinant PvMSP-10 protein. The antigenicity of MSP-10 was analyzed, and we found 42 % sensitivity and 95 % specificity using serum samples from P. vivax-infected Korean patients. The IgG1 and IgG3 were the predominant immunoreactive antibodies against PvMSP-10 in vivax patient sera, and IgG1 and IgG3 and Th1-type cytokines were predominantly secreted in PvMSP-10-immunized mice. We conclude that the immunogenicity and antigenicity of MSP-10 may serve as a potential vaccine against vivax malaria.

Original languageEnglish
Pages (from-to)2559-2568
Number of pages10
JournalParasitology Research
Volume113
Issue number7
DOIs
Publication statusPublished - 2014 Jan 1

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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