Abstract
Pneumocystis pneumonia (PCP) is caused by the yeast-like fungus Pneumocystis jirovecii, which is specific to humans. PCP could be a source of opportunistic infection in adults that are immunosuppressed and children with prematurity or malnutrition. The diagnosis should be confirmed by identification of the causative organism, by analysis of the sputum, a bronchoalveolar lavage or a tissue biopsy. In both histologic and cytologic specimens, the cysts are contained within frothy exudates, which form aggregated clumps. The cysts often collapse forming crescent-shaped bodies that resemble ping-pong balls. We recently diagnosed nine cases of PCP using an immunohistochemical stain for Pneumocystis. The patients consisted of five human immunodefficiency virus positive individuals, two renal transplant recipients, and two patients with a malignant disease. All nine patients were infected with P. jirovecii, which was positive for monoclonal antibody 3F6. In conclusion, the immunohistochemical stain used in this report is a new technique for the detection of P. jirovecii infection.
| Original language | English |
|---|---|
| Pages (from-to) | 115-118 |
| Number of pages | 4 |
| Journal | Korean Journal of Pathology |
| Volume | 45 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2011 Feb 1 |
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Keywords
- Bronchoalveolar lavage
- Immunohistochemistry
- Pneumocystis jirovecii
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Immunohistochemical identification of Pneumocystis jirovecii in liquid-based cytology of bronchoalveolar lavage - Nine cases report. / Lee, Jeong Hyeon; Lee, Ji Young; Shin, Mi Ran; Ahn, Hyeong Kee; Kim, Chul Hwan; Kim, Insun.
In: Korean Journal of Pathology, Vol. 45, No. 1, 01.02.2011, p. 115-118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunohistochemical identification of Pneumocystis jirovecii in liquid-based cytology of bronchoalveolar lavage - Nine cases report
AU - Lee, Jeong Hyeon
AU - Lee, Ji Young
AU - Shin, Mi Ran
AU - Ahn, Hyeong Kee
AU - Kim, Chul Hwan
AU - Kim, Insun
PY - 2011/2/1
Y1 - 2011/2/1
N2 - Pneumocystis pneumonia (PCP) is caused by the yeast-like fungus Pneumocystis jirovecii, which is specific to humans. PCP could be a source of opportunistic infection in adults that are immunosuppressed and children with prematurity or malnutrition. The diagnosis should be confirmed by identification of the causative organism, by analysis of the sputum, a bronchoalveolar lavage or a tissue biopsy. In both histologic and cytologic specimens, the cysts are contained within frothy exudates, which form aggregated clumps. The cysts often collapse forming crescent-shaped bodies that resemble ping-pong balls. We recently diagnosed nine cases of PCP using an immunohistochemical stain for Pneumocystis. The patients consisted of five human immunodefficiency virus positive individuals, two renal transplant recipients, and two patients with a malignant disease. All nine patients were infected with P. jirovecii, which was positive for monoclonal antibody 3F6. In conclusion, the immunohistochemical stain used in this report is a new technique for the detection of P. jirovecii infection.
AB - Pneumocystis pneumonia (PCP) is caused by the yeast-like fungus Pneumocystis jirovecii, which is specific to humans. PCP could be a source of opportunistic infection in adults that are immunosuppressed and children with prematurity or malnutrition. The diagnosis should be confirmed by identification of the causative organism, by analysis of the sputum, a bronchoalveolar lavage or a tissue biopsy. In both histologic and cytologic specimens, the cysts are contained within frothy exudates, which form aggregated clumps. The cysts often collapse forming crescent-shaped bodies that resemble ping-pong balls. We recently diagnosed nine cases of PCP using an immunohistochemical stain for Pneumocystis. The patients consisted of five human immunodefficiency virus positive individuals, two renal transplant recipients, and two patients with a malignant disease. All nine patients were infected with P. jirovecii, which was positive for monoclonal antibody 3F6. In conclusion, the immunohistochemical stain used in this report is a new technique for the detection of P. jirovecii infection.
KW - Bronchoalveolar lavage
KW - Immunohistochemistry
KW - Pneumocystis jirovecii
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U2 - 10.4132/KoreanJPathol.2011.45.1.115
DO - 10.4132/KoreanJPathol.2011.45.1.115
M3 - Article
VL - 45
SP - 115
EP - 118
JO - Journal of Pathology and Translational Medicine
JF - Journal of Pathology and Translational Medicine
SN - 2383-7837
IS - 1
ER -