Immunohistochemical staining for megakaryocyte c-mpl may complememt morphologic distinction between polycythemia vera and secondary erythrocytosis

Ayalew Tefferi, Soo-Young Yoon, Chin Yang Li

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Recent studies have shown decreased megakaryocyte expression of the thrombopoietin receptor (c-mpl) in patients with polycythemia vera (PV) but not in those with reactive erythrocytosis. We examined the diagnostic utility of this observation in 22 patients with PV, 7 patients with secondary erythrocytosis (SE), and 10 normal controls. Commercial antibodies against c- mpl were used with standard immunoperoxidase methods. Megakaryocyte c-mpl staining intensity was uniformly moderate-to-strong in the healthy controls and in all the patients with SE. In contrast, staining intensity in 9 patients with PV (41%) was uniformly weak. Furthermore, in 12 of the remaining 13 patients with PV, the c-mpl staining pattern in each case was heterogeneous and was associated with weak staining intensity in more than 20% of the megakaryocyte population. These preliminary data suggest that c- mpl immunostains may complement bone marrow histopathology in distinguishing PV from non-clonal causes of erythrocytosis. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)771-772
Number of pages2
JournalBlood
Volume96
Issue number2
Publication statusPublished - 2000 Jul 15
Externally publishedYes

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Polycythemia Vera
Polycythemia
Megakaryocytes
Thrombopoietin Receptors
Staining and Labeling
Bone
Antibodies
Bone Marrow
Population

ASJC Scopus subject areas

  • Hematology

Cite this

Immunohistochemical staining for megakaryocyte c-mpl may complememt morphologic distinction between polycythemia vera and secondary erythrocytosis. / Tefferi, Ayalew; Yoon, Soo-Young; Li, Chin Yang.

In: Blood, Vol. 96, No. 2, 15.07.2000, p. 771-772.

Research output: Contribution to journalArticle

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abstract = "Recent studies have shown decreased megakaryocyte expression of the thrombopoietin receptor (c-mpl) in patients with polycythemia vera (PV) but not in those with reactive erythrocytosis. We examined the diagnostic utility of this observation in 22 patients with PV, 7 patients with secondary erythrocytosis (SE), and 10 normal controls. Commercial antibodies against c- mpl were used with standard immunoperoxidase methods. Megakaryocyte c-mpl staining intensity was uniformly moderate-to-strong in the healthy controls and in all the patients with SE. In contrast, staining intensity in 9 patients with PV (41{\%}) was uniformly weak. Furthermore, in 12 of the remaining 13 patients with PV, the c-mpl staining pattern in each case was heterogeneous and was associated with weak staining intensity in more than 20{\%} of the megakaryocyte population. These preliminary data suggest that c- mpl immunostains may complement bone marrow histopathology in distinguishing PV from non-clonal causes of erythrocytosis. (C) 2000 by The American Society of Hematology.",
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