Immunohistochemical staining for megakaryocyte c-mpl may complememt morphologic distinction between polycythemia vera and secondary erythrocytosis

Ayalew Tefferi, Soo Young Yoon, Chin Yang Li

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Recent studies have shown decreased megakaryocyte expression of the thrombopoietin receptor (c-mpl) in patients with polycythemia vera (PV) but not in those with reactive erythrocytosis. We examined the diagnostic utility of this observation in 22 patients with PV, 7 patients with secondary erythrocytosis (SE), and 10 normal controls. Commercial antibodies against c- mpl were used with standard immunoperoxidase methods. Megakaryocyte c-mpl staining intensity was uniformly moderate-to-strong in the healthy controls and in all the patients with SE. In contrast, staining intensity in 9 patients with PV (41%) was uniformly weak. Furthermore, in 12 of the remaining 13 patients with PV, the c-mpl staining pattern in each case was heterogeneous and was associated with weak staining intensity in more than 20% of the megakaryocyte population. These preliminary data suggest that c- mpl immunostains may complement bone marrow histopathology in distinguishing PV from non-clonal causes of erythrocytosis. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)771-772
Number of pages2
JournalBlood
Volume96
Issue number2
DOIs
Publication statusPublished - 2000 Jul 15
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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