Immunohistochemical studies of human ribosomal protein S3 (rpS3)

Soo Hyun Choi, So Young Kim, Jae Jin An, Sun Hwa Lee, Dae Won Kim, Moo Ho Won, Tae Cheon Kang, Jinseu Park, Won Sik Eum, Joon Kim, Soo Young Choi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The human ribosomal protein S3 (rpS3) was expressed in E. coli using the pET-15b vector and the monoclonal antibodies (mAbs) were produced and characterized. A total of five hybridoma cell lines were established and the antibodies recognized a single band of molecular weight of 33 kDa on immunoblot with purified rpS3. When the purified rpS3 was incubated with the mAbs, the UV endonuclease activity of rpS3 was inhibited up to a maximum of 49%. The binding affinity of mAbs to rpS3 determined by using a biosensor technology showed that they have similar binding affinities. Using the anti-rpS3 antibodies as probes, we investigated the cross-reactivities of various other mammalian brain tissues and cell lines, including human. The immunoreactive bands on Western blots appeared to be the same molecular mass of 33 kDa in all animal species tested. They also appear to be extensively cross-reactive among different organs in rat. These results demonstrated that only one type of immuno-logically similar rpS3 protein is present in all of the mammalian brain tissues including human. Furthermore, these antibodies were successfully applied in immuno-histochemistry in order to detect rpS3 in the gerbil brain tissues. Among the various regions in the brain tissues, the rpS3 positive neurons were predominantly observed in the ependymal cells, hippocampus and substantia nigra pars compacta. The different distributions of rpS3 in brain tissues reply that rpS3 protein may play an important second function in the neuronal cells.

Original languageEnglish
Pages (from-to)208-215
Number of pages8
JournalJournal of Biochemistry and Molecular Biology
Volume39
Issue number2
Publication statusPublished - 2006 Mar 1
Externally publishedYes

Fingerprint

Brain
Tissue
Monoclonal Antibodies
Antibodies
Cells
human RPS3 protein
ribosomal protein S3
Cell Line
Gerbillinae
Hybridomas
Molecular mass
Biosensing Techniques
Biosensors
Escherichia coli
Neurons
Rats
Hippocampus
Animals
Proteins
Molecular Weight

Keywords

  • Cross-reactivity
  • Human ribosomal protein S3 (rpS3)
  • Immunohistochemistry
  • Monoclonal Antibodies (mAbs)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Choi, S. H., Kim, S. Y., An, J. J., Lee, S. H., Kim, D. W., Won, M. H., ... Choi, S. Y. (2006). Immunohistochemical studies of human ribosomal protein S3 (rpS3). Journal of Biochemistry and Molecular Biology, 39(2), 208-215.

Immunohistochemical studies of human ribosomal protein S3 (rpS3). / Choi, Soo Hyun; Kim, So Young; An, Jae Jin; Lee, Sun Hwa; Kim, Dae Won; Won, Moo Ho; Kang, Tae Cheon; Park, Jinseu; Eum, Won Sik; Kim, Joon; Choi, Soo Young.

In: Journal of Biochemistry and Molecular Biology, Vol. 39, No. 2, 01.03.2006, p. 208-215.

Research output: Contribution to journalArticle

Choi, SH, Kim, SY, An, JJ, Lee, SH, Kim, DW, Won, MH, Kang, TC, Park, J, Eum, WS, Kim, J & Choi, SY 2006, 'Immunohistochemical studies of human ribosomal protein S3 (rpS3)', Journal of Biochemistry and Molecular Biology, vol. 39, no. 2, pp. 208-215.
Choi SH, Kim SY, An JJ, Lee SH, Kim DW, Won MH et al. Immunohistochemical studies of human ribosomal protein S3 (rpS3). Journal of Biochemistry and Molecular Biology. 2006 Mar 1;39(2):208-215.
Choi, Soo Hyun ; Kim, So Young ; An, Jae Jin ; Lee, Sun Hwa ; Kim, Dae Won ; Won, Moo Ho ; Kang, Tae Cheon ; Park, Jinseu ; Eum, Won Sik ; Kim, Joon ; Choi, Soo Young. / Immunohistochemical studies of human ribosomal protein S3 (rpS3). In: Journal of Biochemistry and Molecular Biology. 2006 ; Vol. 39, No. 2. pp. 208-215.
@article{357b44afe6c64dbd8108ab0df449a1be,
title = "Immunohistochemical studies of human ribosomal protein S3 (rpS3)",
abstract = "The human ribosomal protein S3 (rpS3) was expressed in E. coli using the pET-15b vector and the monoclonal antibodies (mAbs) were produced and characterized. A total of five hybridoma cell lines were established and the antibodies recognized a single band of molecular weight of 33 kDa on immunoblot with purified rpS3. When the purified rpS3 was incubated with the mAbs, the UV endonuclease activity of rpS3 was inhibited up to a maximum of 49{\%}. The binding affinity of mAbs to rpS3 determined by using a biosensor technology showed that they have similar binding affinities. Using the anti-rpS3 antibodies as probes, we investigated the cross-reactivities of various other mammalian brain tissues and cell lines, including human. The immunoreactive bands on Western blots appeared to be the same molecular mass of 33 kDa in all animal species tested. They also appear to be extensively cross-reactive among different organs in rat. These results demonstrated that only one type of immuno-logically similar rpS3 protein is present in all of the mammalian brain tissues including human. Furthermore, these antibodies were successfully applied in immuno-histochemistry in order to detect rpS3 in the gerbil brain tissues. Among the various regions in the brain tissues, the rpS3 positive neurons were predominantly observed in the ependymal cells, hippocampus and substantia nigra pars compacta. The different distributions of rpS3 in brain tissues reply that rpS3 protein may play an important second function in the neuronal cells.",
keywords = "Cross-reactivity, Human ribosomal protein S3 (rpS3), Immunohistochemistry, Monoclonal Antibodies (mAbs)",
author = "Choi, {Soo Hyun} and Kim, {So Young} and An, {Jae Jin} and Lee, {Sun Hwa} and Kim, {Dae Won} and Won, {Moo Ho} and Kang, {Tae Cheon} and Jinseu Park and Eum, {Won Sik} and Joon Kim and Choi, {Soo Young}",
year = "2006",
month = "3",
day = "1",
language = "English",
volume = "39",
pages = "208--215",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "2",

}

TY - JOUR

T1 - Immunohistochemical studies of human ribosomal protein S3 (rpS3)

AU - Choi, Soo Hyun

AU - Kim, So Young

AU - An, Jae Jin

AU - Lee, Sun Hwa

AU - Kim, Dae Won

AU - Won, Moo Ho

AU - Kang, Tae Cheon

AU - Park, Jinseu

AU - Eum, Won Sik

AU - Kim, Joon

AU - Choi, Soo Young

PY - 2006/3/1

Y1 - 2006/3/1

N2 - The human ribosomal protein S3 (rpS3) was expressed in E. coli using the pET-15b vector and the monoclonal antibodies (mAbs) were produced and characterized. A total of five hybridoma cell lines were established and the antibodies recognized a single band of molecular weight of 33 kDa on immunoblot with purified rpS3. When the purified rpS3 was incubated with the mAbs, the UV endonuclease activity of rpS3 was inhibited up to a maximum of 49%. The binding affinity of mAbs to rpS3 determined by using a biosensor technology showed that they have similar binding affinities. Using the anti-rpS3 antibodies as probes, we investigated the cross-reactivities of various other mammalian brain tissues and cell lines, including human. The immunoreactive bands on Western blots appeared to be the same molecular mass of 33 kDa in all animal species tested. They also appear to be extensively cross-reactive among different organs in rat. These results demonstrated that only one type of immuno-logically similar rpS3 protein is present in all of the mammalian brain tissues including human. Furthermore, these antibodies were successfully applied in immuno-histochemistry in order to detect rpS3 in the gerbil brain tissues. Among the various regions in the brain tissues, the rpS3 positive neurons were predominantly observed in the ependymal cells, hippocampus and substantia nigra pars compacta. The different distributions of rpS3 in brain tissues reply that rpS3 protein may play an important second function in the neuronal cells.

AB - The human ribosomal protein S3 (rpS3) was expressed in E. coli using the pET-15b vector and the monoclonal antibodies (mAbs) were produced and characterized. A total of five hybridoma cell lines were established and the antibodies recognized a single band of molecular weight of 33 kDa on immunoblot with purified rpS3. When the purified rpS3 was incubated with the mAbs, the UV endonuclease activity of rpS3 was inhibited up to a maximum of 49%. The binding affinity of mAbs to rpS3 determined by using a biosensor technology showed that they have similar binding affinities. Using the anti-rpS3 antibodies as probes, we investigated the cross-reactivities of various other mammalian brain tissues and cell lines, including human. The immunoreactive bands on Western blots appeared to be the same molecular mass of 33 kDa in all animal species tested. They also appear to be extensively cross-reactive among different organs in rat. These results demonstrated that only one type of immuno-logically similar rpS3 protein is present in all of the mammalian brain tissues including human. Furthermore, these antibodies were successfully applied in immuno-histochemistry in order to detect rpS3 in the gerbil brain tissues. Among the various regions in the brain tissues, the rpS3 positive neurons were predominantly observed in the ependymal cells, hippocampus and substantia nigra pars compacta. The different distributions of rpS3 in brain tissues reply that rpS3 protein may play an important second function in the neuronal cells.

KW - Cross-reactivity

KW - Human ribosomal protein S3 (rpS3)

KW - Immunohistochemistry

KW - Monoclonal Antibodies (mAbs)

UR - http://www.scopus.com/inward/record.url?scp=33645303786&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645303786&partnerID=8YFLogxK

M3 - Article

C2 - 16584637

AN - SCOPUS:33645303786

VL - 39

SP - 208

EP - 215

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 2

ER -