Immunological characterization of 130 kDa phospholipase C-β4 isozyme in rat cerebellar Purkinje cells

Do Sik Min, Myung Jong Kim, Hyun Kyung Jeong, Young Han Lee, Hyun Kim, Hee Sup Shin, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed a monoclonal antibody, β4-22-9 that recognizes an epitope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-β4 (PLC-β4). Tissue distribution of 130 kDa PLC-β4 was determined with this monoclonal antibody. One hundred and thirty kiloDalton PLC-β4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at very high levels in cerebellum. The expression of 130 kDa PLC-β4 in cerebellum was gradually increased from 5 days after birth to adulthood. In the adult cerebellum, especially high level of 130 kDa PLC-β4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data implies that 130 kDa PLC-β4 may a role in cerebellar Purkinje cells. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalNeuroscience Letters
Volume292
Issue number1
DOIs
Publication statusPublished - 2000 Sep 29

Fingerprint

Purkinje Cells
Type C Phospholipases
Isoenzymes
Cerebellum
Monoclonal Antibodies
Nerve Tissue
Cerebrum
Tissue Distribution
Epitopes
Parturition
Amino Acids

Keywords

  • Immunohistochemistry
  • Monoclonal antibody
  • Phosphoinositide-specific phospholipase C
  • Phospholipase C-β4
  • Purkinje cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Immunological characterization of 130 kDa phospholipase C-β4 isozyme in rat cerebellar Purkinje cells. / Min, Do Sik; Kim, Myung Jong; Jeong, Hyun Kyung; Lee, Young Han; Kim, Hyun; Shin, Hee Sup; Ryu, Sung Ho; Suh, Pann Ghill.

In: Neuroscience Letters, Vol. 292, No. 1, 29.09.2000, p. 9-12.

Research output: Contribution to journalArticle

Min, Do Sik ; Kim, Myung Jong ; Jeong, Hyun Kyung ; Lee, Young Han ; Kim, Hyun ; Shin, Hee Sup ; Ryu, Sung Ho ; Suh, Pann Ghill. / Immunological characterization of 130 kDa phospholipase C-β4 isozyme in rat cerebellar Purkinje cells. In: Neuroscience Letters. 2000 ; Vol. 292, No. 1. pp. 9-12.
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AU - Shin, Hee Sup

AU - Ryu, Sung Ho

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