Immunomodulatory effects of balneotherapy with hae-un-dae thermal water on imiquimod-induced psoriasis-like murine model

Young Bok Lee, Jun Young Lee, Hye Jin Lee, Seong Taek Yun, Jong Tae Lee, Hong Jig Kim, Dong Soo Yu, So Youn Woo, Jin Wou Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Balneotherapy, although not a well-established dermatological treatment, is thought to have therapeutic properties for psoriasis and is used as an alternative treatment modality throughout the world. Objective: To evaluate the mechanism underlying the therapeutic immunologic effects of thermomineral water. Methods: A murine model of imiquimod-induced psoriasis-like skin inflammation was used for evaluating the therapeutic effects of balneotherapy with Hae-Un-Dae hot spring mineral water. The clinical improvements were evaluated by a dermatologist. Lesional cytokines, including interleukin (IL)-17A, IL-23, and IL-22, were quantitatively measured by real-time reverse transcriptase polymerase chain reaction. Serum levels of interferon- γ, IL-4, IL-5, and IL-17A were measured by enzyme- linked immunosorbent assay. T cell proportions in the spleen were evaluated by flow cytometry, and histopathological evaluation of the skin was also performed. Results: The mineral water balneotherapy group showed faster improvement in skin erythema and scales than the distilled water bathing group. A substantial reduction was observed in the lesional mRNA levels of IL-17A and IL-23 in the mineral water group. Serum levels of IL-4 and IL-5 were significantly decreased in the mineral water group but not in the distilled water group. Normalized T cell proportions were observed after bathing. Conclusion: Balneotherapy showed immunomodulatory effects in a psoriasis-like murine model. Balneotherapy suppressed lesional IL-23 and IL-17A, which are important cytokines in the pathogenesis of psoriasis. These results suggest that balneotherapy can be used as an effective and safe treatment for psoriasis. (Ann Dermatol 26(2) 221∼230, 2014).

Original languageEnglish
Pages (from-to)221-230
Number of pages10
JournalAnnals of Dermatology
Volume26
Issue number2
DOIs
Publication statusPublished - 2014 Jan 1

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imiquimod
Balneology
Psoriasis
Mineral Waters
Interleukin-17
Hot Temperature
Interleukin-23
Water
Interleukin-5
Therapeutic Uses
Interleukin-4
Skin
Hot Springs
Cytokines
T-Lymphocytes
Therapeutics
Erythema
Reverse Transcriptase Polymerase Chain Reaction
Serum
Interferons

ASJC Scopus subject areas

  • Dermatology

Cite this

Immunomodulatory effects of balneotherapy with hae-un-dae thermal water on imiquimod-induced psoriasis-like murine model. / Lee, Young Bok; Lee, Jun Young; Lee, Hye Jin; Yun, Seong Taek; Lee, Jong Tae; Kim, Hong Jig; Yu, Dong Soo; Woo, So Youn; Kim, Jin Wou.

In: Annals of Dermatology, Vol. 26, No. 2, 01.01.2014, p. 221-230.

Research output: Contribution to journalArticle

Lee, Young Bok ; Lee, Jun Young ; Lee, Hye Jin ; Yun, Seong Taek ; Lee, Jong Tae ; Kim, Hong Jig ; Yu, Dong Soo ; Woo, So Youn ; Kim, Jin Wou. / Immunomodulatory effects of balneotherapy with hae-un-dae thermal water on imiquimod-induced psoriasis-like murine model. In: Annals of Dermatology. 2014 ; Vol. 26, No. 2. pp. 221-230.
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abstract = "Background: Balneotherapy, although not a well-established dermatological treatment, is thought to have therapeutic properties for psoriasis and is used as an alternative treatment modality throughout the world. Objective: To evaluate the mechanism underlying the therapeutic immunologic effects of thermomineral water. Methods: A murine model of imiquimod-induced psoriasis-like skin inflammation was used for evaluating the therapeutic effects of balneotherapy with Hae-Un-Dae hot spring mineral water. The clinical improvements were evaluated by a dermatologist. Lesional cytokines, including interleukin (IL)-17A, IL-23, and IL-22, were quantitatively measured by real-time reverse transcriptase polymerase chain reaction. Serum levels of interferon- γ, IL-4, IL-5, and IL-17A were measured by enzyme- linked immunosorbent assay. T cell proportions in the spleen were evaluated by flow cytometry, and histopathological evaluation of the skin was also performed. Results: The mineral water balneotherapy group showed faster improvement in skin erythema and scales than the distilled water bathing group. A substantial reduction was observed in the lesional mRNA levels of IL-17A and IL-23 in the mineral water group. Serum levels of IL-4 and IL-5 were significantly decreased in the mineral water group but not in the distilled water group. Normalized T cell proportions were observed after bathing. Conclusion: Balneotherapy showed immunomodulatory effects in a psoriasis-like murine model. Balneotherapy suppressed lesional IL-23 and IL-17A, which are important cytokines in the pathogenesis of psoriasis. These results suggest that balneotherapy can be used as an effective and safe treatment for psoriasis. (Ann Dermatol 26(2) 221∼230, 2014).",
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AU - Lee, Jun Young

AU - Lee, Hye Jin

AU - Yun, Seong Taek

AU - Lee, Jong Tae

AU - Kim, Hong Jig

AU - Yu, Dong Soo

AU - Woo, So Youn

AU - Kim, Jin Wou

PY - 2014/1/1

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N2 - Background: Balneotherapy, although not a well-established dermatological treatment, is thought to have therapeutic properties for psoriasis and is used as an alternative treatment modality throughout the world. Objective: To evaluate the mechanism underlying the therapeutic immunologic effects of thermomineral water. Methods: A murine model of imiquimod-induced psoriasis-like skin inflammation was used for evaluating the therapeutic effects of balneotherapy with Hae-Un-Dae hot spring mineral water. The clinical improvements were evaluated by a dermatologist. Lesional cytokines, including interleukin (IL)-17A, IL-23, and IL-22, were quantitatively measured by real-time reverse transcriptase polymerase chain reaction. Serum levels of interferon- γ, IL-4, IL-5, and IL-17A were measured by enzyme- linked immunosorbent assay. T cell proportions in the spleen were evaluated by flow cytometry, and histopathological evaluation of the skin was also performed. Results: The mineral water balneotherapy group showed faster improvement in skin erythema and scales than the distilled water bathing group. A substantial reduction was observed in the lesional mRNA levels of IL-17A and IL-23 in the mineral water group. Serum levels of IL-4 and IL-5 were significantly decreased in the mineral water group but not in the distilled water group. Normalized T cell proportions were observed after bathing. Conclusion: Balneotherapy showed immunomodulatory effects in a psoriasis-like murine model. Balneotherapy suppressed lesional IL-23 and IL-17A, which are important cytokines in the pathogenesis of psoriasis. These results suggest that balneotherapy can be used as an effective and safe treatment for psoriasis. (Ann Dermatol 26(2) 221∼230, 2014).

AB - Background: Balneotherapy, although not a well-established dermatological treatment, is thought to have therapeutic properties for psoriasis and is used as an alternative treatment modality throughout the world. Objective: To evaluate the mechanism underlying the therapeutic immunologic effects of thermomineral water. Methods: A murine model of imiquimod-induced psoriasis-like skin inflammation was used for evaluating the therapeutic effects of balneotherapy with Hae-Un-Dae hot spring mineral water. The clinical improvements were evaluated by a dermatologist. Lesional cytokines, including interleukin (IL)-17A, IL-23, and IL-22, were quantitatively measured by real-time reverse transcriptase polymerase chain reaction. Serum levels of interferon- γ, IL-4, IL-5, and IL-17A were measured by enzyme- linked immunosorbent assay. T cell proportions in the spleen were evaluated by flow cytometry, and histopathological evaluation of the skin was also performed. Results: The mineral water balneotherapy group showed faster improvement in skin erythema and scales than the distilled water bathing group. A substantial reduction was observed in the lesional mRNA levels of IL-17A and IL-23 in the mineral water group. Serum levels of IL-4 and IL-5 were significantly decreased in the mineral water group but not in the distilled water group. Normalized T cell proportions were observed after bathing. Conclusion: Balneotherapy showed immunomodulatory effects in a psoriasis-like murine model. Balneotherapy suppressed lesional IL-23 and IL-17A, which are important cytokines in the pathogenesis of psoriasis. These results suggest that balneotherapy can be used as an effective and safe treatment for psoriasis. (Ann Dermatol 26(2) 221∼230, 2014).

KW - Bath

KW - Immunomodulation

KW - Mineral waters

KW - Psoriasis

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