Immunomodulatory effects of Deokgu thermomineral water balneotherapy on oxazolone-induced atopic dermatitis murine model

Young Bok Lee, Su Jin Kim, Sae Mi Park, Kyung Ho Lee, Hyung Jin Han, Dong Soo Yu, So Youn Woo, Seong Taek Yun, Se Yeong Hamm, Hong Jig Kim, Jin Wou Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Although the therapeutic mechanism of balneotherapy for atopic dermatitis has not been clarified, many atopic patients who visit thermomineral springs have shown clinical improvements. Objective: This study was aimed to evaluate the immunomodulatory effect of thermomineral water balneotherapy on the atopic dermatitis murine model. Methods: The oxazolone-induced atopic dermatitis murine model was used to evaluate the therapeutic effect of balneotherapy with Deokgu thermomineral water compared with distilled water. Histologic evaluation and confocal microscopic imaging were performed to analyze the lesional expression of cluster-of-differentiation (CD)4 and forkhead box p3 (Foxp3). Lesional mRNA expression of interleukin (IL) 33, thymic stromal lymphopoietin (TSLP), and Foxp3 was evaluated by real-time reverse transcription polymerase chain reaction. Results: Compared with the distilled water bath group, confocal microscopic evaluation of CD4 and Foxp3 merged images showed increased expression of regulatory T cells in the thermomineral balneotherapy group. The lesional mRNA level of IL-33 showed a reduced trend in the thermomineral balneotherapy group, whereas the level of mRNA of Foxp3 was increased. TSLP showed a decreased trend in both distilled water and thermomineral water bath groups. There was a trend of reduced expression in lesional IL-33 mRNA but increased cell count of CD4+ Foxp3+ regulatory T cells in thermomineral balneotherapy compared with distilled water bath. Conclusion: Therefore, thermomineral balneotherapy can be an effective and safe adjuvant therapeutic option for atopic dermatitis.

Original languageEnglish
Pages (from-to)192-198
Number of pages7
JournalAnnals of Dermatology
Volume28
Issue number2
DOIs
Publication statusPublished - 2016 Apr 1

Fingerprint

Oxazolone
Balneology
Atopic Dermatitis
Water
Baths
Messenger RNA
Regulatory T-Lymphocytes
Therapeutic Uses
CD4 Lymphocyte Count
Reverse Transcription
Polymerase Chain Reaction
Therapeutics

Keywords

  • Atopic dermatitis
  • Balneotherapy
  • Dermatitis
  • Immunomodulation

ASJC Scopus subject areas

  • Dermatology

Cite this

Immunomodulatory effects of Deokgu thermomineral water balneotherapy on oxazolone-induced atopic dermatitis murine model. / Lee, Young Bok; Kim, Su Jin; Park, Sae Mi; Lee, Kyung Ho; Han, Hyung Jin; Yu, Dong Soo; Woo, So Youn; Yun, Seong Taek; Hamm, Se Yeong; Kim, Hong Jig; Kim, Jin Wou.

In: Annals of Dermatology, Vol. 28, No. 2, 01.04.2016, p. 192-198.

Research output: Contribution to journalArticle

Lee, YB, Kim, SJ, Park, SM, Lee, KH, Han, HJ, Yu, DS, Woo, SY, Yun, ST, Hamm, SY, Kim, HJ & Kim, JW 2016, 'Immunomodulatory effects of Deokgu thermomineral water balneotherapy on oxazolone-induced atopic dermatitis murine model', Annals of Dermatology, vol. 28, no. 2, pp. 192-198. https://doi.org/10.5021/ad.2016.28.2.192
Lee, Young Bok ; Kim, Su Jin ; Park, Sae Mi ; Lee, Kyung Ho ; Han, Hyung Jin ; Yu, Dong Soo ; Woo, So Youn ; Yun, Seong Taek ; Hamm, Se Yeong ; Kim, Hong Jig ; Kim, Jin Wou. / Immunomodulatory effects of Deokgu thermomineral water balneotherapy on oxazolone-induced atopic dermatitis murine model. In: Annals of Dermatology. 2016 ; Vol. 28, No. 2. pp. 192-198.
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abstract = "Background: Although the therapeutic mechanism of balneotherapy for atopic dermatitis has not been clarified, many atopic patients who visit thermomineral springs have shown clinical improvements. Objective: This study was aimed to evaluate the immunomodulatory effect of thermomineral water balneotherapy on the atopic dermatitis murine model. Methods: The oxazolone-induced atopic dermatitis murine model was used to evaluate the therapeutic effect of balneotherapy with Deokgu thermomineral water compared with distilled water. Histologic evaluation and confocal microscopic imaging were performed to analyze the lesional expression of cluster-of-differentiation (CD)4 and forkhead box p3 (Foxp3). Lesional mRNA expression of interleukin (IL) 33, thymic stromal lymphopoietin (TSLP), and Foxp3 was evaluated by real-time reverse transcription polymerase chain reaction. Results: Compared with the distilled water bath group, confocal microscopic evaluation of CD4 and Foxp3 merged images showed increased expression of regulatory T cells in the thermomineral balneotherapy group. The lesional mRNA level of IL-33 showed a reduced trend in the thermomineral balneotherapy group, whereas the level of mRNA of Foxp3 was increased. TSLP showed a decreased trend in both distilled water and thermomineral water bath groups. There was a trend of reduced expression in lesional IL-33 mRNA but increased cell count of CD4+ Foxp3+ regulatory T cells in thermomineral balneotherapy compared with distilled water bath. Conclusion: Therefore, thermomineral balneotherapy can be an effective and safe adjuvant therapeutic option for atopic dermatitis.",
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AU - Park, Sae Mi

AU - Lee, Kyung Ho

AU - Han, Hyung Jin

AU - Yu, Dong Soo

AU - Woo, So Youn

AU - Yun, Seong Taek

AU - Hamm, Se Yeong

AU - Kim, Hong Jig

AU - Kim, Jin Wou

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N2 - Background: Although the therapeutic mechanism of balneotherapy for atopic dermatitis has not been clarified, many atopic patients who visit thermomineral springs have shown clinical improvements. Objective: This study was aimed to evaluate the immunomodulatory effect of thermomineral water balneotherapy on the atopic dermatitis murine model. Methods: The oxazolone-induced atopic dermatitis murine model was used to evaluate the therapeutic effect of balneotherapy with Deokgu thermomineral water compared with distilled water. Histologic evaluation and confocal microscopic imaging were performed to analyze the lesional expression of cluster-of-differentiation (CD)4 and forkhead box p3 (Foxp3). Lesional mRNA expression of interleukin (IL) 33, thymic stromal lymphopoietin (TSLP), and Foxp3 was evaluated by real-time reverse transcription polymerase chain reaction. Results: Compared with the distilled water bath group, confocal microscopic evaluation of CD4 and Foxp3 merged images showed increased expression of regulatory T cells in the thermomineral balneotherapy group. The lesional mRNA level of IL-33 showed a reduced trend in the thermomineral balneotherapy group, whereas the level of mRNA of Foxp3 was increased. TSLP showed a decreased trend in both distilled water and thermomineral water bath groups. There was a trend of reduced expression in lesional IL-33 mRNA but increased cell count of CD4+ Foxp3+ regulatory T cells in thermomineral balneotherapy compared with distilled water bath. Conclusion: Therefore, thermomineral balneotherapy can be an effective and safe adjuvant therapeutic option for atopic dermatitis.

AB - Background: Although the therapeutic mechanism of balneotherapy for atopic dermatitis has not been clarified, many atopic patients who visit thermomineral springs have shown clinical improvements. Objective: This study was aimed to evaluate the immunomodulatory effect of thermomineral water balneotherapy on the atopic dermatitis murine model. Methods: The oxazolone-induced atopic dermatitis murine model was used to evaluate the therapeutic effect of balneotherapy with Deokgu thermomineral water compared with distilled water. Histologic evaluation and confocal microscopic imaging were performed to analyze the lesional expression of cluster-of-differentiation (CD)4 and forkhead box p3 (Foxp3). Lesional mRNA expression of interleukin (IL) 33, thymic stromal lymphopoietin (TSLP), and Foxp3 was evaluated by real-time reverse transcription polymerase chain reaction. Results: Compared with the distilled water bath group, confocal microscopic evaluation of CD4 and Foxp3 merged images showed increased expression of regulatory T cells in the thermomineral balneotherapy group. The lesional mRNA level of IL-33 showed a reduced trend in the thermomineral balneotherapy group, whereas the level of mRNA of Foxp3 was increased. TSLP showed a decreased trend in both distilled water and thermomineral water bath groups. There was a trend of reduced expression in lesional IL-33 mRNA but increased cell count of CD4+ Foxp3+ regulatory T cells in thermomineral balneotherapy compared with distilled water bath. Conclusion: Therefore, thermomineral balneotherapy can be an effective and safe adjuvant therapeutic option for atopic dermatitis.

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KW - Immunomodulation

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