Immunoregulation of macrophages by dynamic ligand presentation via ligand–cation coordination

Heemin Kang, Boguang Yang, Kunyu Zhang, Qi Pan, Weihao Yuan, Gang Li, Liming Bian

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Macrophages regulate host responses to implants through their dynamic adhesion, release, and activation. Herein, we employ bisphosphonate (BP)-coated gold nanoparticle template (BNP) to direct the swift and convertible formation of Mg 2+ -functional Mg 2+ -BP nanoparticle (NP) on the BP-AuNP surface via reversible Mg 2+ -BP coordination, thus producing (Mg 2+ -BP)-Au dimer (MgBNP). Ethylenediaminetetraacetic acid-based Mg 2+ chelation facilitates the dissolution of Mg 2+ -BP NP, thus enabling the reversion of the MgBNP to the BNP. This convertible nanoassembly incorporating cell-adhesive Mg 2+ moieties directs reversible attachment and detachment of macrophages by BP and EDTA, without physical scraping or trypsin that could damage cells. The swift formation of RGD ligand- and Mg 2+ -bifunctional RGD-Mg 2+ -BP NP that yields (RGD-Mg 2+ -BP)-Au dimer (RGDBNP) further stimulates the adhesion and pro-regenerative M2-type polarization of macrophages, both in vitro and in vivo, including rho-associated protein kinase. This swift and non-toxic dimer formation can include diverse bio-functional moieties to regulate host responses to implants.

Original languageEnglish
Article number1696
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

macrophages
Macrophages
Diphosphonates
Ligands
nanoparticles
ligands
ethylenediaminetetraacetic acids
dimers
Nanoparticles
adhesion
trypsin
Dimers
chelation
cells
detachment
adhesives
attachment
Edetic Acid
dissolving
templates

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Immunoregulation of macrophages by dynamic ligand presentation via ligand–cation coordination. / Kang, Heemin; Yang, Boguang; Zhang, Kunyu; Pan, Qi; Yuan, Weihao; Li, Gang; Bian, Liming.

In: Nature communications, Vol. 10, No. 1, 1696, 01.12.2019.

Research output: Contribution to journalArticle

Kang, Heemin ; Yang, Boguang ; Zhang, Kunyu ; Pan, Qi ; Yuan, Weihao ; Li, Gang ; Bian, Liming. / Immunoregulation of macrophages by dynamic ligand presentation via ligand–cation coordination. In: Nature communications. 2019 ; Vol. 10, No. 1.
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