Immunoregulatory properties of a crude extraction fraction rich in polysaccharide from Chrysanthemum zawadskii Herbich var. latilobum and its potential role as a vaccine adjuvant

Jeong Moo Han, Ha Yeon Song, Ho Seong Seo, Eui Hong Byun, Seung Taik Lim, Woo Sik Kim, Eui Baek Byun

Research output: Contribution to journalArticlepeer-review

Abstract

The objective of the current study was to demonstrate the immunostimulatory effects of a polysaccharide isolated from Chrysanthemum zawadskii Herbich var. latilobum leaves (CP) and evaluate its potential as a vaccine adjuvant. Results showed that CP induced maturation of the dendritic cells (DCs). In addition, CP-treated DCs activated naïve T cells to polarized CD4+ and CD8+ T cells and substantially induced the production of IFN-γ and IL-2 in vitro. Furthermore, CP initiated the maturation of DCs via the activation of MAPK and NF-κB signaling pathways. Interestingly, systemic administration of CP-treated DCs pulsed with ovalbumin (OVA) peptides significantly enhanced the immune response in vivo, which included the generation of antigen (OVA)-specific polyfunctional T cells, increased cytotoxic T lymphocyte activity, induction of Th1-mediated humoral immunity, and suppression of tumor growth. Taken together, our study highlighted the immunoregulatory activity of CP as well as its potential as a candidate vaccine adjuvant.

Original languageEnglish
Article number107513
JournalInternational Immunopharmacology
Volume95
DOIs
Publication statusPublished - 2021 Jun

Keywords

  • Adjuvant
  • Anti-tumor immunity
  • Chrysanthemum zawadskii Herbich var. latilobum
  • Dendritic cell
  • Natural product
  • Polyfunctional T cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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