Immunosensor based on the ZnO nanorod networks for the detection of H1N1 swine in?uenza virus

Yunseok Jang, Jungil Park, Youngmi Kim Pak, James Jungho Pak

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

This paper presents an immunosensor fabricated on patterned zinc oxide nanorod networks (ZNNs) for detecting the H1N1 swine in?uenza virus (H1N1 SIV). Nanostructured ZnO with a high isoelectric point (IEP, ∼9.5) possesses good absorbability for proteins with low IEPs. Hydrothermally grown ZNNs were fabricated on a patterned Au electrode (0.02 cm 2) through a lift-off process. To detect the H1N1 SIV, the sandwich enzyme-linked immunosorbent assay (ELISA) method was employed in the immunosensor. The immunosensor was evaluated in an acetate buffer solution containing 3,3′,5,5′- tetramethylbenzidine (TMB) via cyclic voltammetry at various H1N1 SIV concentrations (1 pg/mL-5 ng/mL). The measurement results of the fabricated immunosensor showed that the reduction currents of TMB at 0.25 V logarithmically increased from 259.37 to 577.98 nA as the H1N1 SIV concentration changed from 1 pg/mL to 5 ng/mL. An H1N1 SIV immunosensor, based on the patterned ZNNs, was successfully realized for detecting 1 pg/mL-5 ng/mL H1N1 SIV concentrations, with a detection limit of 1 pg/mL for H1N1 SIV.

Original languageEnglish
Pages (from-to)5173-5177
Number of pages5
JournalJournal of Nanoscience and Nanotechnology
Volume12
Issue number7
DOIs
Publication statusPublished - 2012 Jul

Keywords

  • H1N1 Swine In?uenza Virus
  • Immunosensor
  • ZnO Nanorod Networks

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

Fingerprint

Dive into the research topics of 'Immunosensor based on the ZnO nanorod networks for the detection of H1N1 swine in?uenza virus'. Together they form a unique fingerprint.

Cite this