Immunostimulation activity of a polysaccharide from fermented ginseng with Hericium erinaceum mycelia in solid-state culture

Hoon Kim, Hyung Joo Suh, Ki Han Kwon, Jong Hyun Hwang, Kwang Won Yu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Korean fresh ginseng was cultured with Hericium erinaceum mycelia (HE) in solid-state culture (SSC) to enhance the immunomodulation activity. Hot-water extracts (FG-HE-HW) of H. erinaceum-fermented ginseng (FG-HE) fractionated into a crude polysaccharide (FG-HE-CP) showed higher mitogenic, macrophage stimulation, and intestinal immune system modulation activities (1.52, 1.63, and 1.35x relative to a saline control at 100 μg/mL, respectively) than the crude polysaccharide from non-fermented ginseng (NG-CP; 1.35, 1.50, and 1.18x) and H. erinaceum mycelia alone (HE-CP; 1.26, 1.46, and 1.27x). After FG-HE-CP was further fractionated on a DEAE-Sepharose CL-6B column, the polysaccharide fraction (FG-HE-CP-III) exhibited larger activity increases than any subfraction from NG-CP or HE-CP. FG-HE-CP-III mainly consisted of Ara, Gal, Glc, and GalA (molar ratios of 0.45:1.00: 0.51:0.73). NaIO4 oxidation decreased the activities of FG-HE-CP-III (51.0-76.9%). The polysaccharide from H. erinaceum-fermented ginseng using SSC plays an important role for enhancement of immunomodulation activities of fresh ginseng.

Original languageEnglish
Pages (from-to)311-318
Number of pages8
JournalFood Science and Biotechnology
Volume25
Issue number1
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

immunostimulation (physiological)
Hericium
Panax
Mycelium
mycelium
Polysaccharides
Immunization
polysaccharides
immunomodulation
Immunomodulation
solid state culture
agarose
immune system
Immune System
macrophages
Macrophages
oxidation

Keywords

  • fermented ginseng
  • Hericium erinaceum
  • immunostimulation
  • polysaccharide
  • solid-state culture

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Immunostimulation activity of a polysaccharide from fermented ginseng with Hericium erinaceum mycelia in solid-state culture. / Kim, Hoon; Suh, Hyung Joo; Kwon, Ki Han; Hwang, Jong Hyun; Yu, Kwang Won.

In: Food Science and Biotechnology, Vol. 25, No. 1, 01.02.2016, p. 311-318.

Research output: Contribution to journalArticle

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AB - Korean fresh ginseng was cultured with Hericium erinaceum mycelia (HE) in solid-state culture (SSC) to enhance the immunomodulation activity. Hot-water extracts (FG-HE-HW) of H. erinaceum-fermented ginseng (FG-HE) fractionated into a crude polysaccharide (FG-HE-CP) showed higher mitogenic, macrophage stimulation, and intestinal immune system modulation activities (1.52, 1.63, and 1.35x relative to a saline control at 100 μg/mL, respectively) than the crude polysaccharide from non-fermented ginseng (NG-CP; 1.35, 1.50, and 1.18x) and H. erinaceum mycelia alone (HE-CP; 1.26, 1.46, and 1.27x). After FG-HE-CP was further fractionated on a DEAE-Sepharose CL-6B column, the polysaccharide fraction (FG-HE-CP-III) exhibited larger activity increases than any subfraction from NG-CP or HE-CP. FG-HE-CP-III mainly consisted of Ara, Gal, Glc, and GalA (molar ratios of 0.45:1.00: 0.51:0.73). NaIO4 oxidation decreased the activities of FG-HE-CP-III (51.0-76.9%). The polysaccharide from H. erinaceum-fermented ginseng using SSC plays an important role for enhancement of immunomodulation activities of fresh ginseng.

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