Immunostimulatory effects and characterization of a glycoprotein fraction from rice bran

Ho Young Park, A. Reum Yu, In Wook Choi, Hee Do Hong, Kwang Won Lee, Hee Don Choi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Many natural resources obtained from plants have been studied for their utility as host defense potentiators. In the present study, we investigated whether a glycoprotein fraction from rice (Oryza sativa) bran (GFRB) could modulate immune responses such as the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in the RAW 264.7 macrophage cell line. GFRB, which contained 65.7% of protein and 7.7% of total sugar, was prepared by treating an aqueous extract of rice bran with 80% (NH4)2SO4 and the extraction yield was 4.9%. GFRB consisted of 5 bands with varying molecular weights by SDS-PAGE and remarkably improved production of NO in RAW 264.7 murine macrophage cells, up to approximately 10-fold compared to the normal control at 100 μg/mL concentration. In RAW 264.7 cells treated with 50 μg/mL GFRB, released levels of various cytokines such as TNF-α, IL-1β, IL-6, and IL-10 were 2824.4 ± 90.7, 224.5 ± 4.0, 524.3 ± 4.8, and 143.0 ± 9.5 pg/mL, respectively, which were higher than the levels in normal controls. Moreover, GFRB exhibited no cytotoxicity. According to the results of egion-selective enzyme hydrolysis, the immune responses against GFRB were elicited by the glycans in the GFRB. These results show the potential of GFRB as a functional therapeutic agent with demonstrable immunostimulatory activity.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalInternational Immunopharmacology
Volume17
Issue number2
DOIs
Publication statusPublished - 2013 Aug 20

Fingerprint

Glycoproteins
Interleukin-1
Interleukin-10
Interleukin-6
Nitric Oxide
Tumor Necrosis Factor-alpha
Macrophages
Polysaccharides
Polyacrylamide Gel Electrophoresis
Hydrolysis
Molecular Weight
Cytokines
Enzymes
Oryza
Proteins
RAW 264.7 Cells
Therapeutics
Natural Resources

Keywords

  • Cytokines
  • Glycoprotein
  • Immunostimulatory activity
  • Nitric oxide
  • RAW 264.7 cells
  • Rice bran

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

Immunostimulatory effects and characterization of a glycoprotein fraction from rice bran. / Park, Ho Young; Yu, A. Reum; Choi, In Wook; Hong, Hee Do; Lee, Kwang Won; Choi, Hee Don.

In: International Immunopharmacology, Vol. 17, No. 2, 20.08.2013, p. 191-197.

Research output: Contribution to journalArticle

Park, Ho Young ; Yu, A. Reum ; Choi, In Wook ; Hong, Hee Do ; Lee, Kwang Won ; Choi, Hee Don. / Immunostimulatory effects and characterization of a glycoprotein fraction from rice bran. In: International Immunopharmacology. 2013 ; Vol. 17, No. 2. pp. 191-197.
@article{6e14ea553a4644f6994b128be272b4b5,
title = "Immunostimulatory effects and characterization of a glycoprotein fraction from rice bran",
abstract = "Many natural resources obtained from plants have been studied for their utility as host defense potentiators. In the present study, we investigated whether a glycoprotein fraction from rice (Oryza sativa) bran (GFRB) could modulate immune responses such as the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in the RAW 264.7 macrophage cell line. GFRB, which contained 65.7{\%} of protein and 7.7{\%} of total sugar, was prepared by treating an aqueous extract of rice bran with 80{\%} (NH4)2SO4 and the extraction yield was 4.9{\%}. GFRB consisted of 5 bands with varying molecular weights by SDS-PAGE and remarkably improved production of NO in RAW 264.7 murine macrophage cells, up to approximately 10-fold compared to the normal control at 100 μg/mL concentration. In RAW 264.7 cells treated with 50 μg/mL GFRB, released levels of various cytokines such as TNF-α, IL-1β, IL-6, and IL-10 were 2824.4 ± 90.7, 224.5 ± 4.0, 524.3 ± 4.8, and 143.0 ± 9.5 pg/mL, respectively, which were higher than the levels in normal controls. Moreover, GFRB exhibited no cytotoxicity. According to the results of egion-selective enzyme hydrolysis, the immune responses against GFRB were elicited by the glycans in the GFRB. These results show the potential of GFRB as a functional therapeutic agent with demonstrable immunostimulatory activity.",
keywords = "Cytokines, Glycoprotein, Immunostimulatory activity, Nitric oxide, RAW 264.7 cells, Rice bran",
author = "Park, {Ho Young} and Yu, {A. Reum} and Choi, {In Wook} and Hong, {Hee Do} and Lee, {Kwang Won} and Choi, {Hee Don}",
year = "2013",
month = "8",
day = "20",
doi = "10.1016/j.intimp.2013.06.013",
language = "English",
volume = "17",
pages = "191--197",
journal = "International Immunopharmacology",
issn = "1567-5769",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Immunostimulatory effects and characterization of a glycoprotein fraction from rice bran

AU - Park, Ho Young

AU - Yu, A. Reum

AU - Choi, In Wook

AU - Hong, Hee Do

AU - Lee, Kwang Won

AU - Choi, Hee Don

PY - 2013/8/20

Y1 - 2013/8/20

N2 - Many natural resources obtained from plants have been studied for their utility as host defense potentiators. In the present study, we investigated whether a glycoprotein fraction from rice (Oryza sativa) bran (GFRB) could modulate immune responses such as the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in the RAW 264.7 macrophage cell line. GFRB, which contained 65.7% of protein and 7.7% of total sugar, was prepared by treating an aqueous extract of rice bran with 80% (NH4)2SO4 and the extraction yield was 4.9%. GFRB consisted of 5 bands with varying molecular weights by SDS-PAGE and remarkably improved production of NO in RAW 264.7 murine macrophage cells, up to approximately 10-fold compared to the normal control at 100 μg/mL concentration. In RAW 264.7 cells treated with 50 μg/mL GFRB, released levels of various cytokines such as TNF-α, IL-1β, IL-6, and IL-10 were 2824.4 ± 90.7, 224.5 ± 4.0, 524.3 ± 4.8, and 143.0 ± 9.5 pg/mL, respectively, which were higher than the levels in normal controls. Moreover, GFRB exhibited no cytotoxicity. According to the results of egion-selective enzyme hydrolysis, the immune responses against GFRB were elicited by the glycans in the GFRB. These results show the potential of GFRB as a functional therapeutic agent with demonstrable immunostimulatory activity.

AB - Many natural resources obtained from plants have been studied for their utility as host defense potentiators. In the present study, we investigated whether a glycoprotein fraction from rice (Oryza sativa) bran (GFRB) could modulate immune responses such as the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in the RAW 264.7 macrophage cell line. GFRB, which contained 65.7% of protein and 7.7% of total sugar, was prepared by treating an aqueous extract of rice bran with 80% (NH4)2SO4 and the extraction yield was 4.9%. GFRB consisted of 5 bands with varying molecular weights by SDS-PAGE and remarkably improved production of NO in RAW 264.7 murine macrophage cells, up to approximately 10-fold compared to the normal control at 100 μg/mL concentration. In RAW 264.7 cells treated with 50 μg/mL GFRB, released levels of various cytokines such as TNF-α, IL-1β, IL-6, and IL-10 were 2824.4 ± 90.7, 224.5 ± 4.0, 524.3 ± 4.8, and 143.0 ± 9.5 pg/mL, respectively, which were higher than the levels in normal controls. Moreover, GFRB exhibited no cytotoxicity. According to the results of egion-selective enzyme hydrolysis, the immune responses against GFRB were elicited by the glycans in the GFRB. These results show the potential of GFRB as a functional therapeutic agent with demonstrable immunostimulatory activity.

KW - Cytokines

KW - Glycoprotein

KW - Immunostimulatory activity

KW - Nitric oxide

KW - RAW 264.7 cells

KW - Rice bran

UR - http://www.scopus.com/inward/record.url?scp=84881521177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881521177&partnerID=8YFLogxK

U2 - 10.1016/j.intimp.2013.06.013

DO - 10.1016/j.intimp.2013.06.013

M3 - Article

C2 - 23816537

AN - SCOPUS:84881521177

VL - 17

SP - 191

EP - 197

JO - International Immunopharmacology

JF - International Immunopharmacology

SN - 1567-5769

IS - 2

ER -