Abstract
Many natural resources obtained from plants have been studied for their utility as host defense potentiators. In the present study, we investigated whether a glycoprotein fraction from rice (Oryza sativa) bran (GFRB) could modulate immune responses such as the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in the RAW 264.7 macrophage cell line. GFRB, which contained 65.7% of protein and 7.7% of total sugar, was prepared by treating an aqueous extract of rice bran with 80% (NH4)2SO4 and the extraction yield was 4.9%. GFRB consisted of 5 bands with varying molecular weights by SDS-PAGE and remarkably improved production of NO in RAW 264.7 murine macrophage cells, up to approximately 10-fold compared to the normal control at 100 μg/mL concentration. In RAW 264.7 cells treated with 50 μg/mL GFRB, released levels of various cytokines such as TNF-α, IL-1β, IL-6, and IL-10 were 2824.4 ± 90.7, 224.5 ± 4.0, 524.3 ± 4.8, and 143.0 ± 9.5 pg/mL, respectively, which were higher than the levels in normal controls. Moreover, GFRB exhibited no cytotoxicity. According to the results of egion-selective enzyme hydrolysis, the immune responses against GFRB were elicited by the glycans in the GFRB. These results show the potential of GFRB as a functional therapeutic agent with demonstrable immunostimulatory activity.
| Original language | English |
|---|---|
| Pages (from-to) | 191-197 |
| Number of pages | 7 |
| Journal | International Immunopharmacology |
| Volume | 17 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2013 Aug 20 |
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Keywords
- Cytokines
- Glycoprotein
- Immunostimulatory activity
- Nitric oxide
- RAW 264.7 cells
- Rice bran
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pharmacology
Cite this
Immunostimulatory effects and characterization of a glycoprotein fraction from rice bran. / Park, Ho Young; Yu, A. Reum; Choi, In Wook; Hong, Hee Do; Lee, Kwang Won; Choi, Hee Don.
In: International Immunopharmacology, Vol. 17, No. 2, 20.08.2013, p. 191-197.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunostimulatory effects and characterization of a glycoprotein fraction from rice bran
AU - Park, Ho Young
AU - Yu, A. Reum
AU - Choi, In Wook
AU - Hong, Hee Do
AU - Lee, Kwang Won
AU - Choi, Hee Don
PY - 2013/8/20
Y1 - 2013/8/20
N2 - Many natural resources obtained from plants have been studied for their utility as host defense potentiators. In the present study, we investigated whether a glycoprotein fraction from rice (Oryza sativa) bran (GFRB) could modulate immune responses such as the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in the RAW 264.7 macrophage cell line. GFRB, which contained 65.7% of protein and 7.7% of total sugar, was prepared by treating an aqueous extract of rice bran with 80% (NH4)2SO4 and the extraction yield was 4.9%. GFRB consisted of 5 bands with varying molecular weights by SDS-PAGE and remarkably improved production of NO in RAW 264.7 murine macrophage cells, up to approximately 10-fold compared to the normal control at 100 μg/mL concentration. In RAW 264.7 cells treated with 50 μg/mL GFRB, released levels of various cytokines such as TNF-α, IL-1β, IL-6, and IL-10 were 2824.4 ± 90.7, 224.5 ± 4.0, 524.3 ± 4.8, and 143.0 ± 9.5 pg/mL, respectively, which were higher than the levels in normal controls. Moreover, GFRB exhibited no cytotoxicity. According to the results of egion-selective enzyme hydrolysis, the immune responses against GFRB were elicited by the glycans in the GFRB. These results show the potential of GFRB as a functional therapeutic agent with demonstrable immunostimulatory activity.
AB - Many natural resources obtained from plants have been studied for their utility as host defense potentiators. In the present study, we investigated whether a glycoprotein fraction from rice (Oryza sativa) bran (GFRB) could modulate immune responses such as the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in the RAW 264.7 macrophage cell line. GFRB, which contained 65.7% of protein and 7.7% of total sugar, was prepared by treating an aqueous extract of rice bran with 80% (NH4)2SO4 and the extraction yield was 4.9%. GFRB consisted of 5 bands with varying molecular weights by SDS-PAGE and remarkably improved production of NO in RAW 264.7 murine macrophage cells, up to approximately 10-fold compared to the normal control at 100 μg/mL concentration. In RAW 264.7 cells treated with 50 μg/mL GFRB, released levels of various cytokines such as TNF-α, IL-1β, IL-6, and IL-10 were 2824.4 ± 90.7, 224.5 ± 4.0, 524.3 ± 4.8, and 143.0 ± 9.5 pg/mL, respectively, which were higher than the levels in normal controls. Moreover, GFRB exhibited no cytotoxicity. According to the results of egion-selective enzyme hydrolysis, the immune responses against GFRB were elicited by the glycans in the GFRB. These results show the potential of GFRB as a functional therapeutic agent with demonstrable immunostimulatory activity.
KW - Cytokines
KW - Glycoprotein
KW - Immunostimulatory activity
KW - Nitric oxide
KW - RAW 264.7 cells
KW - Rice bran
UR - http://www.scopus.com/inward/record.url?scp=84881521177&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881521177&partnerID=8YFLogxK
U2 - 10.1016/j.intimp.2013.06.013
DO - 10.1016/j.intimp.2013.06.013
M3 - Article
C2 - 23816537
AN - SCOPUS:84881521177
VL - 17
SP - 191
EP - 197
JO - International Immunopharmacology
JF - International Immunopharmacology
SN - 1567-5769
IS - 2
ER -