Immunostimulatory potential of extracellular vesicles isolated from an edible plant, petasites japonicus, via the induction of murine dendritic cell maturation

Jeong Moo Han, Ha Yeon Song, Seung Taik Lim, Kwang Il Kim, Ho Seong Seo, Eui Baek Byun

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Extracellular vesicles (EVs) have recently been isolated from different plants. Plant-de-rived EVs have been proposed as potent therapeutics and drug-delivery nanoplatforms for deliver-ing biomolecules, including proteins, RNAs, DNAs, and lipids. Herein, Petasites japonicus-derived EVs (PJ-EVs) were isolated through a series of centrifugation steps and characterized using dynamic light scattering and transmission electron microscopy. Immunomodulatory effects of PJ-EVs were assessed using dendritic cells (DCs). PJ-EVs exhibited a spherical morphology with an average size of 122.6 nm. They induced the maturation of DCs via an increase in the expression of surface mole-cules (CD80, CD86, MHC-I, and MHC-II), production of Th1-polarizing cytokines (TNF-α and IL-12p70), and antigen-presenting ability; however, they reduced the antigen-uptake ability. Further-more, maturation of DCs induced by PJ-EVs was dependent on the activation and phosphorylation of MAPK and NF-κB signal pathways. Notably, PJ-EV-treated DCs strongly induced the proliferation and differentiation of naïve T cells toward Th1-type T cells and cytotoxic CD8+ T cells along with robust secretion of IFN-γ and IL-2. In conclusion, our study indicates that PJ-EVs can be potent immunostimulatory candidates with an ability of strongly inducing the maturation of DCs.

Original languageEnglish
Article number10634
JournalInternational journal of molecular sciences
Volume22
Issue number19
DOIs
Publication statusPublished - 2021 Oct 1

Keywords

  • Dendritic cells
  • Extracellular vesicles
  • Immunostimulatory
  • Petasites japonicus
  • T cells

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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