Immunosuppressive mechanisms of embryonic stem cells and mesenchymal stem cells in alloimmune response

Kyu Hyun Han, Han Ro, Ju Ho Hong, Eun Mi Lee, Bumrae Cho, Hye Jung Yeom, Myung-Gyu Kim, Kook Hwan Oh, Curie Ahn, Jaeseok Yang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Although both embryonic stem cells (ESCs) and mesenchymal stem cells (MSCs) are known to have immunosuppressive effects, the mechanisms of immunosuppression are still controversial. Both types of stem cells suppressed not only the proliferation but also survival of CD4+ T cells in vitro. They suppressed secretion of various cytokines (IL-2, IL-12, IFN-γ, TNF-α, IL-4, IL-5, IL-1β, and IL-10), whereas there was no change in the levels of TGF-β or IDO. Classic and modified transwell experiments demonstrated that immunosuppressive activities were mainly mediated by cell-to-cell contact. Granzyme B in the ESCs played a significant role in their immunosuppression, whereas PDL-1, Fas ligand, CD30 or perforin was not involved in the contact-dependent immunosuppression. However, none of the above molecules played a significant role in the immunosuppression by the MSCs. Interestingly, both stem cells increased the proportion of Foxp3+ regulatory T cells. Our results showed that both ESCs and MSCs suppressed the survival as well as the proliferation of T cells by mainly contact-dependent mechanisms and increased the proportion of regulatory T cells. Granzyme B was involved in immunosuppression by the ESCs in a perforin-independent manner.

Original languageEnglish
Pages (from-to)7-15
Number of pages9
JournalTransplant Immunology
Volume25
Issue number1
DOIs
Publication statusPublished - 2011 Jul 1
Externally publishedYes

Fingerprint

Immunosuppressive Agents
Embryonic Stem Cells
Mesenchymal Stromal Cells
Immunosuppression
Granzymes
Perforin
Regulatory T-Lymphocytes
Stem Cells
T-Lymphocytes
Fas Ligand Protein
Interleukin-5
Interleukin-12
Interleukin-1
Interleukin-4
Interleukin-10
Interleukin-2
Cell Survival
Cytokines

Keywords

  • Embryonic stem cells
  • Granzyme B
  • Immunosuppression
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Transplantation

Cite this

Immunosuppressive mechanisms of embryonic stem cells and mesenchymal stem cells in alloimmune response. / Han, Kyu Hyun; Ro, Han; Hong, Ju Ho; Lee, Eun Mi; Cho, Bumrae; Yeom, Hye Jung; Kim, Myung-Gyu; Oh, Kook Hwan; Ahn, Curie; Yang, Jaeseok.

In: Transplant Immunology, Vol. 25, No. 1, 01.07.2011, p. 7-15.

Research output: Contribution to journalArticle

Han, Kyu Hyun ; Ro, Han ; Hong, Ju Ho ; Lee, Eun Mi ; Cho, Bumrae ; Yeom, Hye Jung ; Kim, Myung-Gyu ; Oh, Kook Hwan ; Ahn, Curie ; Yang, Jaeseok. / Immunosuppressive mechanisms of embryonic stem cells and mesenchymal stem cells in alloimmune response. In: Transplant Immunology. 2011 ; Vol. 25, No. 1. pp. 7-15.
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