Immunotoxicity of zinc oxide nanoparticles with different size and electrostatic charge

Cheol Su Kim, Hai Duong Nguyen, Rosa Mistica Ignacio, Jae Hyun Kim, Hyeon Cheol Cho, Eun Ho Maeng, Yu Ri Kim, Meyoung-Kon Kim, Bae Keun Park, Soo Ki Kim

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

While zinc oxide (ZnO) nanoparticles (NPs) have been recognized to have promising applications in biomedicine, their immunotoxicity has been inconsistent and even contradictory. To address this issue, we investigated whether ZnO NPs with different size (20 or 100 nm) and electrostatic charge (positive or negative) would cause immunotoxicity in vitro and in vivo, and explored their underlying molecular mechanism. Using Raw 264.7 cell line, we examined the immunotoxicity mechanism of ZnO NPs as cell viability. We found that in a cell viability assay, ZnO NPs with different size and charge could induce differential cytotoxicity to Raw 264.7 cells. Specifically, the positively charged ZnO NPs exerted higher cytotoxicity than the negatively charged ones. Next, to gauge systemic immunotoxicity, we assessed immune responses of C57BL/6 mice after oral administration of 750 mg/kg/day dose of ZnO NPs for 2 weeks. In parallel, ZnO NPs did not alter the cell-mediated immune response in mice but suppressed innate immunity such as natural killer cell activity. The CD4+/CD8+ ratio, a marker for matured T-cells was slightly reduced, which implies the alteration of immune status induced by ZnO NPs. Accordingly, nitric oxide production from splenocyte culture supernatant in ZnO NP-fed mice was lower than control. Consistently, serum levels of pro/anti-inflammatory (interleukin [IL]-1β, tumor necrosis factor-α, and IL-10) and T helper-1 cytokines (interferon-γ and IL-12p70) in ZnO NP-fed mice were significantly suppressed. Collectively, our results indicate that different sized and charged ZnO NPs would cause in vitro and in vivo immunotoxicity, of which nature is an immunosuppression.

Original languageEnglish
Pages (from-to)195-205
Number of pages11
JournalInternational Journal of Nanomedicine
Volume9
DOIs
Publication statusPublished - 2014 Dec 15

Fingerprint

Zinc Oxide
Zinc oxide
Static Electricity
Nanoparticles
Electrostatics
Cells
Cytotoxicity
Cell Survival
Interferons
CD4-CD8 Ratio
T-cells
Interleukins
Nitric oxide
Interleukin-1
Inbred C57BL Mouse
Innate Immunity
Natural Killer Cells
Interleukin-10
Immunosuppression
Gages

Keywords

  • Cytokine
  • Cytotoxicity
  • Immune response
  • Immunosuppression
  • Innate immunity
  • ZnO

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

Kim, C. S., Nguyen, H. D., Ignacio, R. M., Kim, J. H., Cho, H. C., Maeng, E. H., ... Kim, S. K. (2014). Immunotoxicity of zinc oxide nanoparticles with different size and electrostatic charge. International Journal of Nanomedicine, 9, 195-205. https://doi.org/10.2147/IJN.S57935

Immunotoxicity of zinc oxide nanoparticles with different size and electrostatic charge. / Kim, Cheol Su; Nguyen, Hai Duong; Ignacio, Rosa Mistica; Kim, Jae Hyun; Cho, Hyeon Cheol; Maeng, Eun Ho; Kim, Yu Ri; Kim, Meyoung-Kon; Park, Bae Keun; Kim, Soo Ki.

In: International Journal of Nanomedicine, Vol. 9, 15.12.2014, p. 195-205.

Research output: Contribution to journalArticle

Kim, CS, Nguyen, HD, Ignacio, RM, Kim, JH, Cho, HC, Maeng, EH, Kim, YR, Kim, M-K, Park, BK & Kim, SK 2014, 'Immunotoxicity of zinc oxide nanoparticles with different size and electrostatic charge', International Journal of Nanomedicine, vol. 9, pp. 195-205. https://doi.org/10.2147/IJN.S57935
Kim, Cheol Su ; Nguyen, Hai Duong ; Ignacio, Rosa Mistica ; Kim, Jae Hyun ; Cho, Hyeon Cheol ; Maeng, Eun Ho ; Kim, Yu Ri ; Kim, Meyoung-Kon ; Park, Bae Keun ; Kim, Soo Ki. / Immunotoxicity of zinc oxide nanoparticles with different size and electrostatic charge. In: International Journal of Nanomedicine. 2014 ; Vol. 9. pp. 195-205.
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