Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children

Win Lai May, Myat Phone Kyaw, Stuart D. Blacksell, Sasithon Pukrittayakamee, Kesinee Chotivanich, Borimas Hanboonkunupakarn, Khin Nyo Thein, Chae Seung Lim, Janjira Thaipadungpanit, Thomas Althaus, Podjanee Jittamala

Research output: Contribution to journalArticle

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) deficiency may affect the clinical presentation of dengue due to the altered redox state in immune cells. We aimed to determine the association between G6PD deficiency and severity of dengue infection in paediatric patients in Myanmar. A cross-sectional study was conducted among paediatric patients aged 2–13 years with dengue in Yankin Children Hospital, Myanmar. One hundred and ninety-six patients positive for dengue infection, as determined via PCR or ELISA, were enrolled. Dengue severity was determined according to the 2009 WHO classification guidelines. Spectro-photometric assays determined G6PD levels. The adjusted median G6PD value of males in the study population was used to define various cut-off points according to the WHO classification guidelines. G6PD genotyping for Mahidol, Kaiping and Mediterranean mutations was performed for 128 out of 196 samples by real-time multiplex PCR. 51 of 196 (26.0%) patients had severe dengue. The prevalence of G6PD phenotype deficiency (< 60% activity) in paediatric patients was 14.8% (29/196), specifically, 13.6% (14/103) in males and 16.2% (15/93) in females. Severe deficiency (< 10% activity) accounted for 7.1% (14/196) of our cohort, occurring 11.7% (12/103) in males and 2.2% (2/93) in females. Among 128 samples genotyped, the G6PD gene mutations were detected in 19.5% (25/128) of patients, with 20.3% (13/ 64) in males and 18.8% (12/64) in females. The G6PD Mahidol mutation was 96.0% (24/25) while the G6PD Kaiping mutation was 4.0% (1/25). Severe dengue was not associated with G6PD enzyme deficiency or presence of the G6PD gene mutation. Thus, no association between G6PD deficiency and dengue severity could be detected.

Original languageEnglish
Article numbere0209204
JournalPLoS One
Volume14
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Myanmar
Glucosephosphate Dehydrogenase Deficiency
dengue
Dengue
Glucosephosphate Dehydrogenase
glucose-6-phosphate 1-dehydrogenase
Infection
Mutation
Severe Dengue
Pediatrics
mutation
Guidelines
Multiplex Polymerase Chain Reaction
Genes
Oxidation-Reduction
Real-Time Polymerase Chain Reaction
enzyme deficiencies
Association reactions
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

May, W. L., Kyaw, M. P., Blacksell, S. D., Pukrittayakamee, S., Chotivanich, K., Hanboonkunupakarn, B., ... Jittamala, P. (2019). Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children. PLoS One, 14(1), [e0209204]. https://doi.org/10.1371/journal.pone.0209204

Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children. / May, Win Lai; Kyaw, Myat Phone; Blacksell, Stuart D.; Pukrittayakamee, Sasithon; Chotivanich, Kesinee; Hanboonkunupakarn, Borimas; Thein, Khin Nyo; Lim, Chae Seung; Thaipadungpanit, Janjira; Althaus, Thomas; Jittamala, Podjanee.

In: PLoS One, Vol. 14, No. 1, e0209204, 01.01.2019.

Research output: Contribution to journalArticle

May, WL, Kyaw, MP, Blacksell, SD, Pukrittayakamee, S, Chotivanich, K, Hanboonkunupakarn, B, Thein, KN, Lim, CS, Thaipadungpanit, J, Althaus, T & Jittamala, P 2019, 'Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children', PLoS One, vol. 14, no. 1, e0209204. https://doi.org/10.1371/journal.pone.0209204
May WL, Kyaw MP, Blacksell SD, Pukrittayakamee S, Chotivanich K, Hanboonkunupakarn B et al. Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children. PLoS One. 2019 Jan 1;14(1). e0209204. https://doi.org/10.1371/journal.pone.0209204
May, Win Lai ; Kyaw, Myat Phone ; Blacksell, Stuart D. ; Pukrittayakamee, Sasithon ; Chotivanich, Kesinee ; Hanboonkunupakarn, Borimas ; Thein, Khin Nyo ; Lim, Chae Seung ; Thaipadungpanit, Janjira ; Althaus, Thomas ; Jittamala, Podjanee. / Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children. In: PLoS One. 2019 ; Vol. 14, No. 1.
@article{41e812cce2f94feb929c979b8f29360c,
title = "Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children",
abstract = "Glucose-6-phosphate dehydrogenase (G6PD) deficiency may affect the clinical presentation of dengue due to the altered redox state in immune cells. We aimed to determine the association between G6PD deficiency and severity of dengue infection in paediatric patients in Myanmar. A cross-sectional study was conducted among paediatric patients aged 2–13 years with dengue in Yankin Children Hospital, Myanmar. One hundred and ninety-six patients positive for dengue infection, as determined via PCR or ELISA, were enrolled. Dengue severity was determined according to the 2009 WHO classification guidelines. Spectro-photometric assays determined G6PD levels. The adjusted median G6PD value of males in the study population was used to define various cut-off points according to the WHO classification guidelines. G6PD genotyping for Mahidol, Kaiping and Mediterranean mutations was performed for 128 out of 196 samples by real-time multiplex PCR. 51 of 196 (26.0{\%}) patients had severe dengue. The prevalence of G6PD phenotype deficiency (< 60{\%} activity) in paediatric patients was 14.8{\%} (29/196), specifically, 13.6{\%} (14/103) in males and 16.2{\%} (15/93) in females. Severe deficiency (< 10{\%} activity) accounted for 7.1{\%} (14/196) of our cohort, occurring 11.7{\%} (12/103) in males and 2.2{\%} (2/93) in females. Among 128 samples genotyped, the G6PD gene mutations were detected in 19.5{\%} (25/128) of patients, with 20.3{\%} (13/ 64) in males and 18.8{\%} (12/64) in females. The G6PD Mahidol mutation was 96.0{\%} (24/25) while the G6PD Kaiping mutation was 4.0{\%} (1/25). Severe dengue was not associated with G6PD enzyme deficiency or presence of the G6PD gene mutation. Thus, no association between G6PD deficiency and dengue severity could be detected.",
author = "May, {Win Lai} and Kyaw, {Myat Phone} and Blacksell, {Stuart D.} and Sasithon Pukrittayakamee and Kesinee Chotivanich and Borimas Hanboonkunupakarn and Thein, {Khin Nyo} and Lim, {Chae Seung} and Janjira Thaipadungpanit and Thomas Althaus and Podjanee Jittamala",
year = "2019",
month = "1",
day = "1",
doi = "10.1371/journal.pone.0209204",
language = "English",
volume = "14",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

TY - JOUR

T1 - Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children

AU - May, Win Lai

AU - Kyaw, Myat Phone

AU - Blacksell, Stuart D.

AU - Pukrittayakamee, Sasithon

AU - Chotivanich, Kesinee

AU - Hanboonkunupakarn, Borimas

AU - Thein, Khin Nyo

AU - Lim, Chae Seung

AU - Thaipadungpanit, Janjira

AU - Althaus, Thomas

AU - Jittamala, Podjanee

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Glucose-6-phosphate dehydrogenase (G6PD) deficiency may affect the clinical presentation of dengue due to the altered redox state in immune cells. We aimed to determine the association between G6PD deficiency and severity of dengue infection in paediatric patients in Myanmar. A cross-sectional study was conducted among paediatric patients aged 2–13 years with dengue in Yankin Children Hospital, Myanmar. One hundred and ninety-six patients positive for dengue infection, as determined via PCR or ELISA, were enrolled. Dengue severity was determined according to the 2009 WHO classification guidelines. Spectro-photometric assays determined G6PD levels. The adjusted median G6PD value of males in the study population was used to define various cut-off points according to the WHO classification guidelines. G6PD genotyping for Mahidol, Kaiping and Mediterranean mutations was performed for 128 out of 196 samples by real-time multiplex PCR. 51 of 196 (26.0%) patients had severe dengue. The prevalence of G6PD phenotype deficiency (< 60% activity) in paediatric patients was 14.8% (29/196), specifically, 13.6% (14/103) in males and 16.2% (15/93) in females. Severe deficiency (< 10% activity) accounted for 7.1% (14/196) of our cohort, occurring 11.7% (12/103) in males and 2.2% (2/93) in females. Among 128 samples genotyped, the G6PD gene mutations were detected in 19.5% (25/128) of patients, with 20.3% (13/ 64) in males and 18.8% (12/64) in females. The G6PD Mahidol mutation was 96.0% (24/25) while the G6PD Kaiping mutation was 4.0% (1/25). Severe dengue was not associated with G6PD enzyme deficiency or presence of the G6PD gene mutation. Thus, no association between G6PD deficiency and dengue severity could be detected.

AB - Glucose-6-phosphate dehydrogenase (G6PD) deficiency may affect the clinical presentation of dengue due to the altered redox state in immune cells. We aimed to determine the association between G6PD deficiency and severity of dengue infection in paediatric patients in Myanmar. A cross-sectional study was conducted among paediatric patients aged 2–13 years with dengue in Yankin Children Hospital, Myanmar. One hundred and ninety-six patients positive for dengue infection, as determined via PCR or ELISA, were enrolled. Dengue severity was determined according to the 2009 WHO classification guidelines. Spectro-photometric assays determined G6PD levels. The adjusted median G6PD value of males in the study population was used to define various cut-off points according to the WHO classification guidelines. G6PD genotyping for Mahidol, Kaiping and Mediterranean mutations was performed for 128 out of 196 samples by real-time multiplex PCR. 51 of 196 (26.0%) patients had severe dengue. The prevalence of G6PD phenotype deficiency (< 60% activity) in paediatric patients was 14.8% (29/196), specifically, 13.6% (14/103) in males and 16.2% (15/93) in females. Severe deficiency (< 10% activity) accounted for 7.1% (14/196) of our cohort, occurring 11.7% (12/103) in males and 2.2% (2/93) in females. Among 128 samples genotyped, the G6PD gene mutations were detected in 19.5% (25/128) of patients, with 20.3% (13/ 64) in males and 18.8% (12/64) in females. The G6PD Mahidol mutation was 96.0% (24/25) while the G6PD Kaiping mutation was 4.0% (1/25). Severe dengue was not associated with G6PD enzyme deficiency or presence of the G6PD gene mutation. Thus, no association between G6PD deficiency and dengue severity could be detected.

UR - http://www.scopus.com/inward/record.url?scp=85059502592&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059502592&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0209204

DO - 10.1371/journal.pone.0209204

M3 - Article

VL - 14

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 1

M1 - e0209204

ER -