Impact of low glucose degradation product bicarbonate/lactate-buffered dialysis solution on the epithelial-mesenchymal transition of peritoneum.

Eun Joo Oh, Hye Myung Ryu, Soon Youn Choi, Ju Min Yook, Chan Duck Kim, Sun Hee Park, Ho Young Chung, In San Kim, Min A. Yu, Duk Hee Kang, Yong Lim Kim

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23 Citations (Scopus)

Abstract

BACKGROUND: Epithelial-mesenchymal transition (EMT) is important in the development of peritoneal fibrosis. Glucose degradation products (GDPs) may induce EMT in human peritoneal mesothelial cells (HPMCs). METHODS: The effects of individual GDPs and GDPs derived from peritoneal dialysis fluid (PDF) in both HPMCs and peritoneal membranes were evaluated. EMT was assessed with alpha-smooth muscle actin (alpha-SMA) and E-cadherin. RESULTS: In vitro, alpha-SMA protein and mRNA levels increased in the presence of the GDPs (formaldehyde, glyoxal, methylglyoxal, and 3-deoxyglucosone), and E-cadherin decreased. Changes in the EMT markers were most prominent after exposure to 3-deoxyglucosone. Changes in both alpha-SMA and E-cadherin protein levels were less with low (L)-GDP bicarbonate/lactate-buffered PDF compared to high (H)-GDP PDF. In the rat model after 8 weeks' PDF infusion, the alpha-SMA/E-cadherin mRNA ratio increased in the H-GDP group compared with the L-GDP group (p < 0.05). The peritoneum in the H-GDP group tended to be thicker (p = 0.052) and had more blood vessels than that in the L-GDP group (p < 0.05). Tissue staining for TGF-beta1 decreased in the L-GDP group. Dual-stained cytokeratin and alpha-SMA-positive myofibroblasts in the submesothelial layer were more prominent in the H-GDP group. CONCLUSION: GDPs found in PDF induce EMT of HPMCs, which is associated with peritoneal fibrosis and vascularization. Conversely, L-GDP PDF reduces EMT and peritoneal fibrosis.

Original languageEnglish
Pages (from-to)58-67
Number of pages10
JournalAmerican journal of nephrology
Volume31
Issue number1
DOIs
Publication statusPublished - 2010

ASJC Scopus subject areas

  • Nephrology

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    Oh, E. J., Ryu, H. M., Choi, S. Y., Yook, J. M., Kim, C. D., Park, S. H., Chung, H. Y., Kim, I. S., Yu, M. A., Kang, D. H., & Kim, Y. L. (2010). Impact of low glucose degradation product bicarbonate/lactate-buffered dialysis solution on the epithelial-mesenchymal transition of peritoneum. American journal of nephrology, 31(1), 58-67. https://doi.org/10.1159/000256658