Impacts of GFP-FoxP3+ regulatory T cells on lupus hallmarks differ by genetic background and type of GFP knock-in

Soog Hee Chang, Tae Joo Kim, Yongbaek Kim, Seung Seok Han, Sun Kyung Lee, Ji Hyun Sim, Young Joo Kim, Se Jeong Lee, Im Joo Rhyu, Ki Hoan Nam, Chandra Mohan, Hang Rae Kim

Research output: Contribution to journalArticle

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Abstract

FoxP3 reporter mice expressing green fluorescence protein (GFP) have been used as a very convenient tool to investigate the impact of regulatory T (Treg) cells on pathogenesis in autoimmune diseases. Here, we found that GFP-FoxP3+ knock-in (KI) mice showed alterations in the production of anti-nuclear autoantibodies (ANAs) and nephritis with different extent, depending on the presence or absence of lupus susceptibility gene locus 1 (Sle1) and KI method: contrasting with B6.Sle1.fGFP-FoxP3 mice, expressing GFP via N-terminal insertion, B6.Sle1.iGFP-FoxP3, expressing GFP via bicistronic internal ribosome entry site-driven promotion, exhibited significantly lower penetrance of serum ANA, comparing to control B6.Sle1 mice. Moreover, B6.Sle1.GFP-FoxP3+ mice reduced the Sle1-induced splenomegaly and B-cell expansion independently of the KI method employed, mainly by reducing the numbers of transitional 1 (T1) B cells and CD21CD23 B cells, including plasmablasts and plasma cells. The absolute numbers of both splenic CD4+ T cells and Treg cells from B6.Sle1.GFP-FoxP3 KI mice were significantly reduced but their proportion was not changed, compared to B6.Sle1 mice. Although the glomerular basement membranes were thickened in both B6.Sle1 and B6.Sle1.iGFP-FoxP3 mice, they were thinner in B6.Sle1.fGFP-FoxP3 mice. The latter mice expressed more nephrophilic autoantibodies and deposited more complement component 3 in glomeruli compared to B6.iGFP-FoxP3 mice. FoxP3+ Treg cells may modulate B-cell tolerance in lupus-prone B6.Sle1 mice, presumably by modulating pathogenic, nephrophilic autoantibody production and nephritis.

Original languageEnglish
JournalAutoimmunity
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Regulatory T-Lymphocytes
Fluorescence
Proteins
Autoantibodies
B-Lymphocytes
Nephritis
Genetic Background
Glomerular Basement Membrane
Complement C3
B-Lymphoid Precursor Cells
Penetrance
Splenomegaly
Plasma Cells
Autoimmune Diseases
T-Lymphocytes

Keywords

  • anti-nuclear antibodies
  • green fluorescence protein
  • lupus
  • Regulatory T cells
  • Sle1 gene locus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Impacts of GFP-FoxP3+ regulatory T cells on lupus hallmarks differ by genetic background and type of GFP knock-in. / Chang, Soog Hee; Kim, Tae Joo; Kim, Yongbaek; Han, Seung Seok; Lee, Sun Kyung; Sim, Ji Hyun; Kim, Young Joo; Lee, Se Jeong; Rhyu, Im Joo; Nam, Ki Hoan; Mohan, Chandra; Kim, Hang Rae.

In: Autoimmunity, 01.01.2019.

Research output: Contribution to journalArticle

Chang, Soog Hee ; Kim, Tae Joo ; Kim, Yongbaek ; Han, Seung Seok ; Lee, Sun Kyung ; Sim, Ji Hyun ; Kim, Young Joo ; Lee, Se Jeong ; Rhyu, Im Joo ; Nam, Ki Hoan ; Mohan, Chandra ; Kim, Hang Rae. / Impacts of GFP-FoxP3+ regulatory T cells on lupus hallmarks differ by genetic background and type of GFP knock-in. In: Autoimmunity. 2019.
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AU - Han, Seung Seok

AU - Lee, Sun Kyung

AU - Sim, Ji Hyun

AU - Kim, Young Joo

AU - Lee, Se Jeong

AU - Rhyu, Im Joo

AU - Nam, Ki Hoan

AU - Mohan, Chandra

AU - Kim, Hang Rae

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