Implementation of random bacterial genomic DNA microarray chip (RBGDMC) for screening of dominant bacteria in complex cultures

Byoung Chan Kim, Ji Hyun Park, Man Bock Gu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The random bacterial genomic DNA microarray chip (RBGDMC), which was fabricated using random genomic DNA fragments obtained from the fragmentation of bacterial genome by using four different pairs of restriction enzymes, was found to discriminate bacterial species in the same genus and resulted in the determination of dominant bacteria in enriched cultures. The identification of a dominant bacterial species was successfully conducted in the co-culture of three different bacteria using the RBGDMC. In addition, the analysis of the chip data could confirm if any of the selected bacteria is the most abundant or if some bacteria were enriched and became the dominant species within the consortium after the samples were prepared from the repeated cultures of real sludge in a complex medium. This study shows the successful implementation of the RBGDMC for the identification and monitoring of dominant bacteria in complex environmental bacterial communities simply without any PCR amplification of the target nucleic acids.

Original languageEnglish
Pages (from-to)2284-2293
Number of pages10
JournalApplied Biochemistry and Biotechnology
Volume162
Issue number8
DOIs
Publication statusPublished - 2010 Dec

Keywords

  • Bacteria identification
  • Complex culture
  • DNA microarray chip
  • Genomic DNA
  • Random fragmentation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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