Implication of a small GTPase Rac1 in the activation of c-Jun N-terminal kinase and heat shock factor in response to heat shock

Iy Han Song, Young O. Su, Hyeok Woo Sang, Hee Kim Kyung, Jae-Hong Kim, Do Kim Han, Sung Kang Ho

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Heat shock induces c-Jun N-terminal kinase (JNK) activation as well as heat shock protein (HSP) expression through activation of the heat shock factor (HSF), but its signal pathway is not clearly understood. Since a small GTPase Rac1 has been suggested to participate in the cellular response to stresses, we examined whether Rac1 is involved in the heat shock response. Here we show that moderate heat shock (39-41 °C) induces membrane translocation of Rac1 and membrane ruffling in a Rac1-dependent manner. In addition, Rac1N17, a dominant negative mutant of Rac1, significantly inhibited JNK activation by heat shock. Since Rac1V12 was able to activate JNK, it is suggested that heat shock may activate JNK via Rac1. Similar inhibition by Rac1N17 of HSF activation in response to heat shock was observed. However, inhibitory effects of Rac1N17 on heat shock-induced JNK and HSF activation were reduced as the heat shock temperature increased. Rac1N17 also inhibited HSF activation by L-azetidine-2-carboxylic acid, a proline analog, and heavy metals (CdCl2), suggesting that Rac1 may be linked to HSF activation by denaturation of polypeptides in response to various proteotoxic stresses. However, Rac1N17 did not prevent phosphorylation of HSF1 in response to these proteotoxic stresses. Interestingly, a constitutively active mutant Rac1V12 did not activate the HSF. Therefore, Rac1 activation may be necessary, but not sufficient, for heat shock-inducible HSF activation and HSP expression, or otherwise a signal pathway(s) involving Rac1 may be indirectly involved in the HSF activation. In sum, we suggest that Rac1 may play a critical role(s) in several aspects of the heat shock response.

Original languageEnglish
Pages (from-to)1889-1895
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number3
DOIs
Publication statusPublished - 2001 Jan 19
Externally publishedYes

Fingerprint

Heat-Shock Response
Monomeric GTP-Binding Proteins
JNK Mitogen-Activated Protein Kinases
Shock
Hot Temperature
Chemical activation
Heat-Shock Proteins
Signal Transduction
Azetidinecarboxylic Acid
Cadmium Chloride
Membranes
Heavy Metals
Phosphorylation
Denaturation
Proline

ASJC Scopus subject areas

  • Biochemistry

Cite this

Implication of a small GTPase Rac1 in the activation of c-Jun N-terminal kinase and heat shock factor in response to heat shock. / Song, Iy Han; Su, Young O.; Sang, Hyeok Woo; Kyung, Hee Kim; Kim, Jae-Hong; Han, Do Kim; Ho, Sung Kang.

In: Journal of Biological Chemistry, Vol. 276, No. 3, 19.01.2001, p. 1889-1895.

Research output: Contribution to journalArticle

Song, Iy Han ; Su, Young O. ; Sang, Hyeok Woo ; Kyung, Hee Kim ; Kim, Jae-Hong ; Han, Do Kim ; Ho, Sung Kang. / Implication of a small GTPase Rac1 in the activation of c-Jun N-terminal kinase and heat shock factor in response to heat shock. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 3. pp. 1889-1895.
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AU - Kim, Jae-Hong

AU - Han, Do Kim

AU - Ho, Sung Kang

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