Abstract
Exposure of mammalian cells to ultraviolet (UV) light elicits a cellular response and also lead to apoptotic cell death. However, the role of Rac, a member of Rho family GTPases, in the UV-induced apoptosis has never been examined. In UV-irradiated Rat-2 fibroblasts, nuclear fragmentation began to be observed within 2 h and the total viability of Rat-2 cells were only about 15% at 6 h following by UV irradiation, whereas the total viability in Rat2-RacN17 cells stably expressing RacN17, a dominant negative Rac1 mutant, was almost close to 67%. Pretreatment with SB203580, a specific inhibitor of p38 kinase, likewise attenuated UV-induced cell death, but PD98059, a MEK inhibitor, did not. Thus, Rac1 and p38 kinase appear to be components in the apoptotic signaling pathway induced by UV irradiation in Rat-2 fibroblasts. In addition, our results show that p38 kinase stimulation by UV is dramatically inhibited by RacN17, suggesting that p38 kinase is situated downstream of Rac1 in the UV signaling to apoptosis.
| Original language | English |
|---|---|
| Pages (from-to) | 825-829 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 285 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2001 Oct 14 |
| Externally published | Yes |
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Keywords
- Apoptosis
- p38 kinase
- Rac
- UV
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Implication of the small GTPase Rac1 in the apoptosis induced by UV in Rat-2 fibroblasts. / Eom, Young W.; Yoo, Min Hyuk; Woo, Chang Hoon; Hwang, Ki Chul; Song, Woo Keun; Yoo, Yung Joon; Chun, Jang Soo; Kim, Jae-Hong.
In: Biochemical and Biophysical Research Communications, Vol. 285, No. 3, 14.10.2001, p. 825-829.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Implication of the small GTPase Rac1 in the apoptosis induced by UV in Rat-2 fibroblasts
AU - Eom, Young W.
AU - Yoo, Min Hyuk
AU - Woo, Chang Hoon
AU - Hwang, Ki Chul
AU - Song, Woo Keun
AU - Yoo, Yung Joon
AU - Chun, Jang Soo
AU - Kim, Jae-Hong
PY - 2001/10/14
Y1 - 2001/10/14
N2 - Exposure of mammalian cells to ultraviolet (UV) light elicits a cellular response and also lead to apoptotic cell death. However, the role of Rac, a member of Rho family GTPases, in the UV-induced apoptosis has never been examined. In UV-irradiated Rat-2 fibroblasts, nuclear fragmentation began to be observed within 2 h and the total viability of Rat-2 cells were only about 15% at 6 h following by UV irradiation, whereas the total viability in Rat2-RacN17 cells stably expressing RacN17, a dominant negative Rac1 mutant, was almost close to 67%. Pretreatment with SB203580, a specific inhibitor of p38 kinase, likewise attenuated UV-induced cell death, but PD98059, a MEK inhibitor, did not. Thus, Rac1 and p38 kinase appear to be components in the apoptotic signaling pathway induced by UV irradiation in Rat-2 fibroblasts. In addition, our results show that p38 kinase stimulation by UV is dramatically inhibited by RacN17, suggesting that p38 kinase is situated downstream of Rac1 in the UV signaling to apoptosis.
AB - Exposure of mammalian cells to ultraviolet (UV) light elicits a cellular response and also lead to apoptotic cell death. However, the role of Rac, a member of Rho family GTPases, in the UV-induced apoptosis has never been examined. In UV-irradiated Rat-2 fibroblasts, nuclear fragmentation began to be observed within 2 h and the total viability of Rat-2 cells were only about 15% at 6 h following by UV irradiation, whereas the total viability in Rat2-RacN17 cells stably expressing RacN17, a dominant negative Rac1 mutant, was almost close to 67%. Pretreatment with SB203580, a specific inhibitor of p38 kinase, likewise attenuated UV-induced cell death, but PD98059, a MEK inhibitor, did not. Thus, Rac1 and p38 kinase appear to be components in the apoptotic signaling pathway induced by UV irradiation in Rat-2 fibroblasts. In addition, our results show that p38 kinase stimulation by UV is dramatically inhibited by RacN17, suggesting that p38 kinase is situated downstream of Rac1 in the UV signaling to apoptosis.
KW - Apoptosis
KW - p38 kinase
KW - Rac
KW - UV
UR - http://www.scopus.com/inward/record.url?scp=0034810415&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034810415&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2001.5233
DO - 10.1006/bbrc.2001.5233
M3 - Article
VL - 285
SP - 825
EP - 829
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 3
ER -