Improved cordycepin production by Cordyceps militaris KYL05 using casein hydrolysate in submerged conditions

Soo Kweon Lee, Ju Hun Lee, Hyeong Ryeol Kim, Youngsang Chun, Ja Hyun Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Cordycepin, a beneficial bioactive product specifically found in Cordyceps, has received attention in various bioindustrial applications such as in pharmaceuticals, functional foods, and cosmetics, due to its significant functions. However, lowproductivity of cordycepin is a barrier to commercialization. in this study, Cordyceps militaris was mutated by UV irradiation to improve the cordycepin production. the highest producer KYL05 strain was finally selected and its cordycepin production was increased about 1.5-fold compared to wild type. in addition, the effects of culture conditions were fundamentally investigated. Optimal conditions were as follows: PH 6, temperature of 25°C, shaking speed of 150 rpm, andculture time of 6days. Effects ofmediumcomponent on cordycepinproductionwere also investigated by using various carbon and nitrogen sources. It was found that glucose and casein hydrolysate (CH) weremost effective as carbon and nitrogen sources in cordycepin production (2.3-fold improvement)with maximum cordycepin production of about 445 mg/L. in particular, production was significantly affected by CH. These results should be of value in improving the efficiency of mass production of cordycepin.

Original languageEnglish
Article number461
Issue number9
Publication statusPublished - 2019 Sept


  • Casein hydrolysate (CH)
  • Cordycepin
  • Cordyceps militaris
  • Submerged culture

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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