Abstract
Cordycepin, a beneficial bioactive product specifically found in Cordyceps, has received attention in various bioindustrial applications such as in pharmaceuticals, functional foods, and cosmetics, due to its significant functions. However, low productivity of cordycepin is a barrier to commercialization. In this study, Cordyceps militaris was mutated by UV irradiation to improve the cordycepin production. The highest producer KYL05 strain was finally selected and its cordycepin production was increased about 1.5-fold compared to wild type. In addition, the effects of culture conditions were fundamentally investigated. Optimal conditions were as follows: pH 6, temperature of 25 °C, shaking speed of 150 rpm, and culture time of 6 days. Effects of medium component on cordycepin production were also investigated by using various carbon and nitrogen sources. It was found that glucose and casein hydrolysate (CH) were most effective as carbon and nitrogen sources in cordycepin production (2.3-fold improvement) with maximum cordycepin production of about 445 mg/L. In particular, production was significantly affected by CH. These results should be of value in improving the efficiency of mass production of cordycepin.
| Original language | English |
|---|---|
| Journal | Biomolecules |
| Volume | 9 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2019 Sep 7 |
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Keywords
- casein hydrolysate (CH)
- cordycepin
- Cordyceps militaris
- submerged culture
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
Improved Cordycepin Production by Cordyceps militaris KYL05 Using Casein Hydrolysate in Submerged Conditions. / Lee, Soo Kweon; Lee, Ju Hun; Kim, Hyeong Ryeol; Chun, Youngsang; Lee, Ja Hyun; Yoo, Hah Young; Park, Chulhwan; Kim, Seung Wook.
In: Biomolecules, Vol. 9, No. 9, 07.09.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved Cordycepin Production by Cordyceps militaris KYL05 Using Casein Hydrolysate in Submerged Conditions
AU - Lee, Soo Kweon
AU - Lee, Ju Hun
AU - Kim, Hyeong Ryeol
AU - Chun, Youngsang
AU - Lee, Ja Hyun
AU - Yoo, Hah Young
AU - Park, Chulhwan
AU - Kim, Seung Wook
PY - 2019/9/7
Y1 - 2019/9/7
N2 - Cordycepin, a beneficial bioactive product specifically found in Cordyceps, has received attention in various bioindustrial applications such as in pharmaceuticals, functional foods, and cosmetics, due to its significant functions. However, low productivity of cordycepin is a barrier to commercialization. In this study, Cordyceps militaris was mutated by UV irradiation to improve the cordycepin production. The highest producer KYL05 strain was finally selected and its cordycepin production was increased about 1.5-fold compared to wild type. In addition, the effects of culture conditions were fundamentally investigated. Optimal conditions were as follows: pH 6, temperature of 25 °C, shaking speed of 150 rpm, and culture time of 6 days. Effects of medium component on cordycepin production were also investigated by using various carbon and nitrogen sources. It was found that glucose and casein hydrolysate (CH) were most effective as carbon and nitrogen sources in cordycepin production (2.3-fold improvement) with maximum cordycepin production of about 445 mg/L. In particular, production was significantly affected by CH. These results should be of value in improving the efficiency of mass production of cordycepin.
AB - Cordycepin, a beneficial bioactive product specifically found in Cordyceps, has received attention in various bioindustrial applications such as in pharmaceuticals, functional foods, and cosmetics, due to its significant functions. However, low productivity of cordycepin is a barrier to commercialization. In this study, Cordyceps militaris was mutated by UV irradiation to improve the cordycepin production. The highest producer KYL05 strain was finally selected and its cordycepin production was increased about 1.5-fold compared to wild type. In addition, the effects of culture conditions were fundamentally investigated. Optimal conditions were as follows: pH 6, temperature of 25 °C, shaking speed of 150 rpm, and culture time of 6 days. Effects of medium component on cordycepin production were also investigated by using various carbon and nitrogen sources. It was found that glucose and casein hydrolysate (CH) were most effective as carbon and nitrogen sources in cordycepin production (2.3-fold improvement) with maximum cordycepin production of about 445 mg/L. In particular, production was significantly affected by CH. These results should be of value in improving the efficiency of mass production of cordycepin.
KW - casein hydrolysate (CH)
KW - cordycepin
KW - Cordyceps militaris
KW - submerged culture
UR - http://www.scopus.com/inward/record.url?scp=85071983840&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071983840&partnerID=8YFLogxK
U2 - 10.3390/biom9090461
DO - 10.3390/biom9090461
M3 - Article
C2 - 31500325
AN - SCOPUS:85071983840
VL - 9
JO - Biomolecules
JF - Biomolecules
SN - 2218-273X
IS - 9
ER -