Improved DNA chip with poly(amidoamine) dendrimer peripherally modified with biotin and avidin

Sung Bin Lim, Kyung Woo Kim, Chang Woo Lee, Hyo Sun Kim, Chang Soo Lee, Min-Kyu Oh

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity.

Original languageEnglish
Pages (from-to)683-689
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Volume13
Issue number6
DOIs
Publication statusPublished - 2008 Dec 1

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Keywords

  • Avidin-biotin conjugation
  • DNAchip
  • Fluorescence
  • PAMAM dendrimer

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Bioengineering

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