Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment

Jin Seop Bak, Ja Kyong Ko, Young Hwan Han, Byung Cheol Lee, In-Geol Choi, Kyoung Heon Kim

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

Rice straw was irradiated using an electron beam at currents and then hydrolyzed with cellulase and β-glucosidase to produce glucose. The pretreatment by electron beam irradiation (EBI) was found to significantly increase the enzyme digestibility of rice straw. Specifically, when rice straw that was pretreated by EBI at 80 kGy at 0.12 mA and 1 MeV was hydrolyzed with 60 FPU of cellulase and 30 CBU of β-glucosidase, the glucose yield after 132 h of hydrolysis was 52.1% of theoretical maximum. This value was significantly higher than the 22.6% that was obtained when untreated rice straw was used. In addition, SEM analysis of pretreated rice straw revealed that EBI caused apparent damage to the surface of the rice straw. Furthermore, EBI pretreatment was found to increase the crystalline portion of the rice straw. Finally, the crystallinity and enzyme digestibility were found to be strongly correlated between rice straw samples that were pretreated by EBI under different conditions.

Original languageEnglish
Pages (from-to)1285-1290
Number of pages6
JournalBioresource Technology
Volume100
Issue number3
DOIs
Publication statusPublished - 2009 Feb 1

Fingerprint

Enzymatic hydrolysis
Straw
straw
Electron beams
hydrolysis
irradiation
rice
Irradiation
electron
Glucosidases
Cellulase
digestibility
Glucose
glucose
Enzymes
enzyme
crystallinity
Hydrolysis
scanning electron microscopy
Crystalline materials

Keywords

  • Biofuel
  • Biomass
  • Electron beam irradiation
  • Lignocellulose
  • Pretreatment

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment. / Bak, Jin Seop; Ko, Ja Kyong; Han, Young Hwan; Lee, Byung Cheol; Choi, In-Geol; Kim, Kyoung Heon.

In: Bioresource Technology, Vol. 100, No. 3, 01.02.2009, p. 1285-1290.

Research output: Contribution to journalArticle

Bak, Jin Seop ; Ko, Ja Kyong ; Han, Young Hwan ; Lee, Byung Cheol ; Choi, In-Geol ; Kim, Kyoung Heon. / Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment. In: Bioresource Technology. 2009 ; Vol. 100, No. 3. pp. 1285-1290.
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AU - Han, Young Hwan

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AU - Choi, In-Geol

AU - Kim, Kyoung Heon

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