Improved functionality of fermented milk is mediated by the synbiotic interaction between Cudrania tricuspidata leaf extract and Lactobacillus gasseri strains

Nam Su Oh, Ji Young Lee, Sangnam Oh, Jae Yeon Joung, Su Gyeong Kim, Yong Kook Shin, Kwang Won Lee, Sae Hun Kim, Younghoon Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This study was designed to investigate the cooperative effect of selected Lactobacillus gasseri strains and Cudrania tricuspidata (CT) leaf extract in enhancing the health-promoting activities of fermented milk. Addition of CT increased total bacterial counts and proteolysis during fermentation of milk with L. gasseri strains. Antioxidant capacities were determined by measuring the ABTS, DPPH, and peroxyl radical scavenging activities and ferric reducing power. The antioxidant capacity of CT-supplemented milk was greater than that of milk without supplementation; moreover, the antioxidant activity of CT-supplemented milk was synergistically improved by fermentation with L. gasseri strains. In particular, CT-supplemented milk fermented by L. gasseri 505 showed the highest antioxidant activity. The phenolic compounds in CT, such as neo-chlorogenic, chlorogenic, and caffeic acid, were metabolized during fermentation with L. gasseri strains, and 3,4-dihydroxy-hydrocinnamic acid was produced as a fermentation metabolite. Moreover, the liberation of bioactive peptides of fermented milk was increased by the proteolytic activity of L. gasseri strains. In particular, six peptides, which were mainly derived from β-casein, were newly identified in this study. These findings suggest that L. gasseri strains metabolize the phenolic acids in the CT and the bioactive peptides released through this interaction improve the antioxidant activity of the fermented milk.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalApplied Microbiology and Biotechnology
DOIs
Publication statusAccepted/In press - 2016 Mar 21

Keywords

  • Antioxidant activity
  • Cudrania tricuspidata
  • Lactobacillus gasseri
  • Milk peptide
  • Phenolics metabolism

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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