Improved production of isobutanol in pervaporation-coupled bioreactor using sugarcane bagasse hydrolysate in engineered Enterobacter aerogenes

Hwi Min Jung, Ju Yeon Lee, Jung-hyun Lee, Min-Kyu Oh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A process of isobutanol production from sugarcane bagasse hydrolysates in Enterobacter aerogenes was developed here with a pervaporation-integrated procedure. Isobutanol pathway was overexpressed in a mutant strain with eliminated byproduct-forming enzymes (LdhA, BudA, and PflB). A glucose-and-xylose–coconsuming ptsG mutant was constructed for effective utilization of lignocellulosic biomass. Toxic effects of isobutanol were alleviated by in situ recovery via a pervaporation procedure. Compared to single-batch fermentation, cell growth and isobutanol titer were improved by 60% and 100%, respectively, in the pervaporation-integrated fermentation process. A lab-made cross-linked polydimethylsiloxane membrane was cast on polyvinylidene fluoride and used in the pervaporation process. The membrane-penetrating condensate contained 55–226 g m−2 h−1 isobutanol with 6–25 g L−1 ethanol after separation. This study offers improved fermentative production of isobutanol from lignocellulosic biomass with a pervaporation procedure.

Original languageEnglish
Pages (from-to)373-380
Number of pages8
JournalBioresource Technology
Volume259
DOIs
Publication statusPublished - 2018 Jul 1

Keywords

  • Enterobacter aerogenes
  • Isobutanol
  • Lignocellulosic biomass
  • Pervaporation
  • Sugarcane bagasse hydrolysate

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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