Improved production of N-acetylglucosamine in Saccharomyces cerevisiae by reducing glycolytic flux

Sang Woo Lee, Min-Kyu Oh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Glucosamine and its derivatives are utilized in the food and biomedical industries. However, current production relies on hydrolysis of natural sources, making it difficult to maintain quality and eliminate allergenic risk. Therefore, microbial production with aid of metabolic engineering is required. We previously demonstrated production of N-acetylglucosamine (GlcNAc) in Saccharomyces cerevisiae by overexpressing an allosteric regulation-free Gfa1p mutant and the haloacid dehalogenase-like phosphatase YqaB. In this study, we further improved GlcNAc production by reducing glycolytic flux. Eukaryotic phosphofructokinase 1 (PFK-1) is allosterically activated by fructose 2,6-bisphosphate (F26BP). Disruption of PFK-2, which synthesizes F26BP, resulted in a slight decrease of GlcNAc production and no significant change of glucose consumption and ethanol production. However, when galactose was used as a sole carbon source to the strain without PFK-2, GlcNAc production was significantly increased and ethanol production was reduced, suggesting that further reduction of glycolytic flux can be used to further improve GlcNAc production. The methodology used in this study can be applied to improve production of carbohydrate derivatives in S. cerevisiae. Biotechnol. Bioeng. Biotechnol. Bioeng. 2016;113: 2524–2528.

Original languageEnglish
Pages (from-to)2524-2528
Number of pages5
JournalBiotechnology and Bioengineering
Volume113
Issue number11
DOIs
Publication statusPublished - 2016 Nov 1

Keywords

  • metabolic engineering
  • N-acetylglucosamine
  • phosphofructokinase
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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