Improved spinal fusion efficacy by long-term delivery of bone morphogenetic protein-2 in a rabbit model

Jae Wook Lee, Saehyoung Lee, Sun Hwa Lee, Hee Seok Yang, Gun II Im, Chang Sung Kim, Jung-Ho Park, Byung Soo Kim

Research output: Contribution to journalArticle

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Abstract

Background and purpose: Various new delivery systems for recombinant human bone morphogenetic protein-2 (rhBMP-2) have been introduced to improve its efficacy in osteogenesis. Of these, we have previously developed heparin-conjugated PLGA nanospheres (HCPN) as a long-term delivery system for BMP-2. In vitro studies have shown that the BMP-2 long-term delivery system enhances the level of bone formation. However, the long-term effects of BMP-2 on spinal fusion have not been assessed. Therefore, we now tested the hypothesis that the long-term delivery of BMP-2 using HCPN improves spinal fusion compared to short-term delivery in a rabbit fusion model. Methods: 24 adult New Zealand White rabbits underwent posterolateral fusion (6 animals in 4 groups). The autograft group received an autologous iliac chip bone graft as a positive control. The BMP-2-PN group received rhBMP-2 (20 μg per implant) and PLGA nanospheres (PN) suspended in fibrin gel, and served as a short-term release group. The HCPN group received HCPN suspended in fibrin gel without BMP-2 as a negative control. The BMP-2-HCPN group received rhBMP-2 (20 μg per implant)-bound HCPN suspended in fibrin gel and served as a long-term release group. All animals were killed 12 weeks after surgery. Manual palpation, axial tensile tests, radiography, and histological evaluations were then performed. Results: The spinal fusion rate and Young's modulus of the fusion mass were better in the BMP-2 long-term delivery group than in the short-term delivery group at an equivalent dose. However, the outcome of the long-term delivery was inferior to that of the autograft group. Interpretation: The HCPN system showed potential as an effective carrier that might improve the osteogenic efficacy of BMP-2 for spinal fusion. Copyright:

Original languageEnglish
Pages (from-to)756-760
Number of pages5
JournalActa Orthopaedica
Volume82
Issue number6
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Nanospheres
Bone Morphogenetic Protein 2
Spinal Fusion
Rabbits
Heparin
Fibrin
Gels
Autografts
Osteogenesis
polylactic acid-polyglycolic acid copolymer
Palpation
Elastic Modulus
Radiography
Transplants
Bone and Bones

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Lee, J. W., Lee, S., Lee, S. H., Yang, H. S., Im, G. II., Kim, C. S., ... Kim, B. S. (2011). Improved spinal fusion efficacy by long-term delivery of bone morphogenetic protein-2 in a rabbit model. Acta Orthopaedica, 82(6), 756-760. https://doi.org/10.3109/17453674.2011.636675

Improved spinal fusion efficacy by long-term delivery of bone morphogenetic protein-2 in a rabbit model. / Lee, Jae Wook; Lee, Saehyoung; Lee, Sun Hwa; Yang, Hee Seok; Im, Gun II; Kim, Chang Sung; Park, Jung-Ho; Kim, Byung Soo.

In: Acta Orthopaedica, Vol. 82, No. 6, 01.12.2011, p. 756-760.

Research output: Contribution to journalArticle

Lee, Jae Wook ; Lee, Saehyoung ; Lee, Sun Hwa ; Yang, Hee Seok ; Im, Gun II ; Kim, Chang Sung ; Park, Jung-Ho ; Kim, Byung Soo. / Improved spinal fusion efficacy by long-term delivery of bone morphogenetic protein-2 in a rabbit model. In: Acta Orthopaedica. 2011 ; Vol. 82, No. 6. pp. 756-760.
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