Improvement of analytical method for pymetrozine in citrus fruits

Jun Ho Jeon, Su Hyun Chun, Min Hyuk Kim, Mi Ok Kim, Kwang Won Lee

Research output: Contribution to journalArticle

Abstract

It is difficult to analyze pymetrozine in citrus fruits using the hydromatrix method because of its low efficiency of purification and overlap of matrix and pymetrozine peaks. Liquid-liquid extraction can analyze pymetrozine in citrus fruits using dichloromethane. Since low pH interferes with the extraction of pymetrozine, the extracts of citrus fruits were maintained over pH 7.0 by adding borax buffer and 1 N NaOH in the improved method. According to the improved method, citrus fruits (such as lemon, lime, orange, tangerine, and grapefruit) were extracted and purified for HPLC-photo diode array analysis. The results of validation were as follows: 4.360 µg/kg of limit of detection, 14.533 µg/kg of limit of quantitation, and 0.007 mg/kg of method quantitative limit. Citrus fruits spiked with pymetrozine showed a recovery range from 71.8 to 83.7% and a coefficient of variation below 6%. Thus, the improved method can efficiently analyze pymetrozine in citrus fruits.

Original languageEnglish
Pages (from-to)316-323
Number of pages8
JournalKorean Journal of Food Science and Technology
Volume51
Issue number4
DOIs
Publication statusPublished - 2019 Jan 1

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Keywords

  • Analytical method
  • Citrus fruits
  • HPLC-PDA
  • Liquid-liquid extraction
  • Pymetrozine

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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