Improvement of coenzyme Q10 production by increasing the NADH/NAD+ ratio in agrobacterium tumefaciens

Bong Seong Koo, Yun Jung Gong, Sang Yong Kim, Chan Wha Kim, Hyeon Cheol Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Previously screened CoQ10-overproducing Agrobacterium tumefaciens A603-35 showed a relatively high NADH/NAD+ ratio (1.1), as compared to parental strain C58 (0.2) when we increased the expression levels of NADH-generating enzymes. Also, the intracellular NADH/NAD+ ratio showed a positive correlation with the CoQ10 content in A603-35. Overexpression of glyceraldehyde 3-phosphate dehydrogenase in A603-35 shifted the NADH/NAD+ ratio at 48 h from 0.8 to 1.2, and thus the CoQ 10 content in flask culture increased from 2.16 to 3.63 mg/g DCW. Due to the addition of hydroxybutyrate to the culture media, the intracellular NADH/NAD+ ratio in A603-35-gapA shifted from 1.2 to 1.4, which led to an increase CoQ10 content (5.27 mg/g DCW).

Original languageEnglish
Pages (from-to)895-898
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number4
DOIs
Publication statusPublished - 2010

Keywords

  • Agrobacterium tumefaciens
  • Coenzyme Q
  • Fermentation
  • Glyceraldehyde 3-phosphate dehydrogenase
  • NADH/NAD ratio

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Improvement of coenzyme Q<sub>10</sub> production by increasing the NADH/NAD<sup>+</sup> ratio in agrobacterium tumefaciens'. Together they form a unique fingerprint.

  • Cite this