Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae

Kyung Ok Yu, Ju Jung, Ahmad Bazli Ramzi, Seung Wook Kim, Chulhwan Park, Sung Ok Han

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The conversion of low-priced glycerol to higher value products has been proposed as a way to improve the economic viability of the biofuels industry. In a previous study, the conversion of glycerol to ethanol in a metabolically engineered strain of Saccharomyces cerevisiae was accomplished by minimizing the synthesis of glycerol, the main by-product in ethanol fermentation processing. To further improve ethanol production, overexpression of the native genes involved in conversion of pyruvate to ethanol in S. cerevisiae was successfully accomplished. The overexpression of an alcohol dehydrogenase (adh1) and a pyruvate decarboxylase (pdc1) caused an increase in growth rate and glycerol consumption under fermentative conditions, which led to a slight increase of the final ethanol yield. The overall expression of the adh1 and pdc1 genes in the modified strains, combined with the lack of the fps1 and gpd2 genes, resulted in a 1.4-fold increase (about 5.4 g/L ethanol produced) in fps1 Δgpd2Δ(pGcyaDak, pGupCas) (about 4.0 g/L ethanol produced). In summary, it is possible to improve the ethanol yield by overexpression of the genes involved in the conversion of pyruvate to ethanol in engineered S. cerevisiae using glycerol as substrate.

Original languageEnglish
Pages (from-to)856-865
Number of pages10
JournalApplied Biochemistry and Biotechnology
Volume166
Issue number4
DOIs
Publication statusPublished - 2012 Feb 1

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Keywords

  • Alcohol dehydrogenase
  • Ethanol production
  • Glycerol
  • Pyruvate decarboxylase
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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