Improvement of Euglena gracilis paramylon production through a cocultivation strategy with the indole-3-acetic acidproducing bacterium Vibrio natriegens

Jee Young Kim, Jeong Joo Oh, Min Seo Jeon, Gyu Hyeok Kim, Yoon E. Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We investigated the putative effects on the growth and paramylon production of Euglena gracilis of cocultivation with Vibrio natriegens. E. gracilis heterotrophically cocultivated with V. natriegens displayed significant increases in biomass productivity and paramylon content. In addition, the effects of the bacterial inoculum density and the timing of inoculation on the growth of E. gracilis were examined, to determine the optimal conditions for cocultivation. With the optimal deployment of V. natriegens, biomass productivity and paramylon content were increased by more than 20% and 35%, respectively, compared to those in axenic E. gracilis cultures. Interestingly, indole-3-acetic acid biosynthesized by V. natriegens was responsible for these enhancements of E. gracilis. The morphology of cocultured E. gracilis cells was assessed. Paramylon granules extracted from the cocultivation were significantly larger than those from axenic culture. Our study showed that screening for appropriate bacteria and subsequent cocultivation with E. gracilis represented an effective way to enhance biomass and metabolite production.

Original languageEnglish
Article numbere01548-19
JournalApplied and environmental microbiology
Volume85
Issue number19
DOIs
Publication statusPublished - 2019 Oct 1

Keywords

  • Cocultivation
  • Euglena gracilis
  • IAA
  • Paramylon
  • Vibrio natriegens

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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