TY - JOUR
T1 - Improvement of hydrocarbon recovery by spouting solvent into culture of botryococcus braunii
AU - Choi, Seung Phill
AU - Bahn, Sang Hoon
AU - Sim, Sang Jun
N1 - Funding Information:
Acknowledgments This study was supported by the grant from Korea CCS R&D Center (Grant No. 2011-0031997) and University– Institute cooperation program (2012) of the National Research Foundation of Korea (NRF) funded by the Korean Government (MEST). This work has been also supported by the Korea District Heating Corporation as a part of the Project of ‘‘Development of the technology for CO2 reduction in flue gas and biodiesel production by microalgae using closed system’’ (1003542011S037) in ‘‘New Renewable Energy Technology R&D’’ project, and by DVS Korea Ltd.
PY - 2013/11
Y1 - 2013/11
N2 - Botryococcus braunii, a green microalga, is known to produce plentiful liquid hydrocarbons as promising biodiesel resources. However, the hydrocarbon extraction methods that have so far achieved have several problems such as low efficiency and high cost. In our study, a solvent-spouted extraction process integrated with photo-bioculture was designed for simultaneous realization of hydrocarbon extraction and cell culture in two phases. The n-octane was selected as the best solvent among several solvents because its biocompatibility was highest for B. braunii. As a result, high level of biomass and hydrocarbon, 4.17 and 893.79 mg/L, respectively, was attained at 100 mL/min of solvent recycling rate through three times of processes for 66 days. Moreover, formation of cell clump was suppressed in solvent extraction, cells were regenerated after it, and thus cell viability was maintained even after repeated cycles of it. Finally, this solventspouted culture process required the smaller cost due to reuse of the less solvent and regenerated cells, compared with the other conventional methods. Accordingly, this technique would be applicable to exploit the continuous extraction of hydrocarbon from the algal biomass, especially for application on a large scale.
AB - Botryococcus braunii, a green microalga, is known to produce plentiful liquid hydrocarbons as promising biodiesel resources. However, the hydrocarbon extraction methods that have so far achieved have several problems such as low efficiency and high cost. In our study, a solvent-spouted extraction process integrated with photo-bioculture was designed for simultaneous realization of hydrocarbon extraction and cell culture in two phases. The n-octane was selected as the best solvent among several solvents because its biocompatibility was highest for B. braunii. As a result, high level of biomass and hydrocarbon, 4.17 and 893.79 mg/L, respectively, was attained at 100 mL/min of solvent recycling rate through three times of processes for 66 days. Moreover, formation of cell clump was suppressed in solvent extraction, cells were regenerated after it, and thus cell viability was maintained even after repeated cycles of it. Finally, this solventspouted culture process required the smaller cost due to reuse of the less solvent and regenerated cells, compared with the other conventional methods. Accordingly, this technique would be applicable to exploit the continuous extraction of hydrocarbon from the algal biomass, especially for application on a large scale.
KW - Botryococcus braunii
KW - Cell regeneration
KW - Hydrocarbon
KW - N-octane
KW - Solvent extraction
UR - http://www.scopus.com/inward/record.url?scp=84891316356&partnerID=8YFLogxK
U2 - 10.1007/s00449-013-0974-7
DO - 10.1007/s00449-013-0974-7
M3 - Article
C2 - 23703677
AN - SCOPUS:84891316356
SN - 1615-7591
VL - 36
SP - 1977
EP - 1985
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
IS - 12
ER -