Improvement of regio-specific production of myricetin-3-O-α-L- rhamnoside in engineered Escherichia coli

Nguyen Huy Thuan, Ramesh Prasad Pandey, Ta Thi Thu Thuy, Je Won Park, Jae Kyung Sohng

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Myricetin is an important flavonol whose medically important properties include activities as an antioxidant, anticarcinogen, and antimutagen. The solubility, stability, and other biological properties of the compounds can be enhanced by conjugating aglycon with sugar moieties. The type of sugar moiety also plays a significant role in the biological and physical properties of the natural product glycosides. Reconstructed Escherichia coli containing thymidine diphosphate-α-L-rhamnose sugar gene cassette and Arabidopsis-derived glycosyltransferase were used for rhamnosylation of myricetin. Myricetin (100 μM) was exogenously supplemented to induced cultures of engineered E. coli. The formation of target product - myricetin-3-O-α-L-rhamnoside - was confirmed by chromatographic and NMR analyses. The yield of product was improved by using various mutants and methylated cyclodextrin as a molecular carrier for myricetin in combination with E. coli M3G3. The maximal yield of product is 55.6 μM (3.31-fold higher than the control E. coli MG3) and shows 55.6 % bioconversion of substrate under optimized conditions.

Original languageEnglish
Pages (from-to)1956-1967
Number of pages12
JournalApplied Biochemistry and Biotechnology
Volume171
Issue number8
DOIs
Publication statusPublished - 2013 Dec 1
Externally publishedYes

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Keywords

  • Myricetin
  • Myricetin-3-O-α-L-rhamnoside
  • Whole-cell biocatalysis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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