Improvement of the two-dimensional gel electrophoresis analysis for the proteome study of Halobacterium salinarum

Chang Won Cho, So Hee Lee, Jiyeon Choi, Soo Jin Park, Dong Jin Ha, Hyo Jeong Kim, Chan Wha Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Inherent problems exist in the use of two-dimensional gel electrophoresis (2-DE) for sample preparation and separation of proteins from Halobacterium salinarum. In particular, proteins from cells grown in 25% NaCl are difficult to resolve by 2-DE due to the abundance of salt. To remove salts, a 3 kDa molecular weight cut-off column was used. When soluble proteins were separated by 2-DE, most of the proteins were concentrated in the acidic range. For separation of proteins in the pH 3-6 range, ultrazoom immobilized pH gradient strips were used. In addition, sample separation using a IPG-phor/Multiphor combined system was a more effective method for the proteome analysis of acidic proteins than using IPGphor for the isoelectric focusing step.

Original languageEnglish
Pages (from-to)2325-2329
Number of pages5
JournalProteomics
Volume3
Issue number12
DOIs
Publication statusPublished - 2003 Dec 1

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Keywords

  • Acidic protein
  • Halobacterium salinarum
  • IPGphor
  • Multiphor combined system
  • Two-dimensional gel electrophoresis
  • Ultrazoom immobilized pH gradient strip

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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