Improving pneumovirus isolation using a centrifugation and AZD1480 combined method

Hansaem Lee, Hye Min Woo, Kisoon Kim, Sehee Park, Man Seong Park, Sung Soon Kim, You Jin Kim

Research output: Contribution to journalArticle

Abstract

The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.

Original languageEnglish
Pages (from-to)2006-2013
Number of pages8
JournalJournal of microbiology and biotechnology
Volume29
Issue number12
DOIs
Publication statusPublished - 2019

Keywords

  • AZD1480
  • Centrifugation
  • Human metapneumovirus
  • Human respiratory syncytial virus
  • Virus isolation

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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    Lee, H., Woo, H. M., Kim, K., Park, S., Park, M. S., Kim, S. S., & Kim, Y. J. (2019). Improving pneumovirus isolation using a centrifugation and AZD1480 combined method. Journal of microbiology and biotechnology, 29(12), 2006-2013. https://doi.org/10.4014/jmb.1906.06050