TY - JOUR
T1 - Improving pneumovirus isolation using a centrifugation and AZD1480 combined method
AU - Lee, Hansaem
AU - Woo, Hye Min
AU - Kim, Kisoon
AU - Park, Sehee
AU - Park, Man Seong
AU - Kim, Sung Soon
AU - Kim, You Jin
N1 - Funding Information:
We acknowledge Dr. Makoto Takeda for kindly providing the recombinant HMPV-GFP. This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National Institute of Health, Korea Centers for Diseases Control and Prevention.
Funding Information:
This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National I nstitute of Health, Korea Centers for Diseases Control and Prevention.
Publisher Copyright:
Copyright© 2019 by The Korean Society for Microbiology and Biotechnology.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2019
Y1 - 2019
N2 - The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.
AB - The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.
KW - AZD1480
KW - Centrifugation
KW - Human metapneumovirus
KW - Human respiratory syncytial virus
KW - Virus isolation
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U2 - 10.4014/jmb.1906.06050
DO - 10.4014/jmb.1906.06050
M3 - Article
C2 - 31581384
AN - SCOPUS:85077348137
VL - 29
SP - 2006
EP - 2013
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
SN - 1017-7825
IS - 12
ER -
