In-situ and highly sensitive detection of epidermal growth factor receptor mutation using nano-porous quartz crystal microbalance

Hyunjun Park, Juneseok You, Chanho Park, Kuewhan Jang, Sung Soo Na

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Assessment of epidermal growth factor receptor (EGFR) exon 19 mutations is very important indicator for treating lung cancer patients. In this report, we propose in-situ and highly sensitive detection method of EGFR mutation using quartz crystal microbalance (QCM) and nano-porous structured QCM electrode. The detection is based on the resonance frequency shift of QCM electrode upon DNA hybridization between probe DNA and EGFR mutant DNA (746–750 (del ELREA) in EGFR exon 19). Nano-porous structure is produced on the QCM electrode through simple processes of electrochemical deposition and etching. By using QCM, in-situ detection of EGFR mutation is performed within 30 mins. High sensitivity is achieved by introducing nano-porous structure on a QCM electrode, and the limit of detection (LOD) is 1 nM.

Original languageEnglish
Pages (from-to)1927-1932
Number of pages6
JournalJournal of Mechanical Science and Technology
Volume32
Issue number4
DOIs
Publication statusPublished - 2018 Apr 1

Keywords

  • Epidermal growth factor receptor (EGFR)
  • In-situ detection
  • Mutation
  • Nano-porous
  • Quartz crystal microbalance (QCM)

ASJC Scopus subject areas

  • Mechanics of Materials
  • Mechanical Engineering

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