Abstract
Objective - The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1α-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results - Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α-derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1α grafts. Conclusions - SDF-1α and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.
| Original language | English |
|---|---|
| Pages (from-to) | E117-E134 |
| Journal | Arteriosclerosis, Thrombosis, and Vascular Biology |
| Volume | 38 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2018 Jul 1 |
Fingerprint
Keywords
- endothelial cells
- regeneration
- stem cells
- substance P
- tissue engineering
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts. / Shafiq, Muhammad; Zhang, Qiuying; Zhi, Dengke; Wang, Kai; Kong, Deling; Kim, Dong-Hwee; Kim, Soo Hyun.
In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 38, No. 7, 01.07.2018, p. E117-E134.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts
AU - Shafiq, Muhammad
AU - Zhang, Qiuying
AU - Zhi, Dengke
AU - Wang, Kai
AU - Kong, Deling
AU - Kim, Dong-Hwee
AU - Kim, Soo Hyun
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Objective - The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1α-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results - Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α-derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1α grafts. Conclusions - SDF-1α and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.
AB - Objective - The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1α-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results - Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α-derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1α grafts. Conclusions - SDF-1α and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.
KW - endothelial cells
KW - regeneration
KW - stem cells
KW - substance P
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85054811503&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054811503&partnerID=8YFLogxK
U2 - 10.1161/ATVBAHA.118.310934
DO - 10.1161/ATVBAHA.118.310934
M3 - Article
C2 - 29853570
AN - SCOPUS:85054811503
VL - 38
SP - E117-E134
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
SN - 1079-5642
IS - 7
ER -