In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering

Sang Soo Han; Hong Yeol Yoon; Ji Young Yhee; Myeong Ok Cho; Hye Eun Shim; Ji Eun Jeong; Dong Eun Lee; Kwangmeyung Kim; Hwanuk Guim; John Hwan Lee; Kang Moo Huh; Sun Woong Kang

doi:10.1039/c7py01654a

In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering

Sang Soo Han, Hong Yeol Yoon, Ji Young Yhee, Myeong Ok Cho, Hye Eun Shim, Ji Eun Jeong, Dong Eun Lee, Kwangmeyung Kim, Hwanuk Guim, John Hwan Lee, Kang Moo Huh, Sun Woong Kang

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

We report a biocompatible and in situ cross-linkable hydrogel derived from hyaluronic acid via a bioorthogonal reaction and confirm the clinical potential of our hydrogel through in vivo cartilage regeneration. Gelation is attributed to copper-free click reactions between an azide and dibenzyl cyclooctyne. HA-PEG4-DBCO was synthesized and cross-linked via 4-arm PEG azide. The effects of the ratio of HA-PEG4-DBCO to 4-arm PEG azide on the gelation time, microstructure, surface morphology, equilibrium swelling, and compressive modulus were examined. The potential of a hydrogel as an injectable scaffold was demonstrated by the encapsulation of chondrocytes within the hydrogel matrix in vitro and in vivo. The results demonstrated that the hydrogel supported cell survival, and the cells regenerated cartilaginous tissue. In addition, these characteristics provide potential opportunities for the use of injectable hydrogels in tissue engineering applications.

Original language	English
Pages (from-to)	20-27
Number of pages	8
Journal	Polymer Chemistry
Volume	9
Issue number	1
DOIs	https://doi.org/10.1039/c7py01654a
Publication status	Published - 2018 Jan 7
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
Biochemistry
Polymers and Plastics
Organic Chemistry

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title = "In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering",

abstract = "We report a biocompatible and in situ cross-linkable hydrogel derived from hyaluronic acid via a bioorthogonal reaction and confirm the clinical potential of our hydrogel through in vivo cartilage regeneration. Gelation is attributed to copper-free click reactions between an azide and dibenzyl cyclooctyne. HA-PEG4-DBCO was synthesized and cross-linked via 4-arm PEG azide. The effects of the ratio of HA-PEG4-DBCO to 4-arm PEG azide on the gelation time, microstructure, surface morphology, equilibrium swelling, and compressive modulus were examined. The potential of a hydrogel as an injectable scaffold was demonstrated by the encapsulation of chondrocytes within the hydrogel matrix in vitro and in vivo. The results demonstrated that the hydrogel supported cell survival, and the cells regenerated cartilaginous tissue. In addition, these characteristics provide potential opportunities for the use of injectable hydrogels in tissue engineering applications.",

author = "Han, {Sang Soo} and Yoon, {Hong Yeol} and Yhee, {Ji Young} and Cho, {Myeong Ok} and Shim, {Hye Eun} and Jeong, {Ji Eun} and Lee, {Dong Eun} and Kwangmeyung Kim and Hwanuk Guim and Lee, {John Hwan} and Huh, {Kang Moo} and Kang, {Sun Woong}",

note = "Funding Information: This study was supported by the INNOPOLIS Foundation grant (2016-02-DD-016), the Bio & Medical Technology Development Program of the National Research Foundation (NRF-2016 M3A9B4919616) funded by the Korean government (Ministry of Science, ICT & Future Planning). We thank Eun Hee Han of the Korea Basic Science Institute (Daejeon, Republic of Korea) for her technical support in the confocal microscopy analysis.",

year = "2018",

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doi = "10.1039/c7py01654a",

language = "English",

volume = "9",

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journal = "Polymer Chemistry",

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T1 - In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering

AU - Han, Sang Soo

AU - Yoon, Hong Yeol

AU - Yhee, Ji Young

AU - Cho, Myeong Ok

AU - Shim, Hye Eun

AU - Jeong, Ji Eun

AU - Lee, Dong Eun

AU - Kim, Kwangmeyung

AU - Guim, Hwanuk

AU - Lee, John Hwan

AU - Huh, Kang Moo

AU - Kang, Sun Woong

N1 - Funding Information: This study was supported by the INNOPOLIS Foundation grant (2016-02-DD-016), the Bio & Medical Technology Development Program of the National Research Foundation (NRF-2016 M3A9B4919616) funded by the Korean government (Ministry of Science, ICT & Future Planning). We thank Eun Hee Han of the Korea Basic Science Institute (Daejeon, Republic of Korea) for her technical support in the confocal microscopy analysis.

PY - 2018/1/7

Y1 - 2018/1/7

N2 - We report a biocompatible and in situ cross-linkable hydrogel derived from hyaluronic acid via a bioorthogonal reaction and confirm the clinical potential of our hydrogel through in vivo cartilage regeneration. Gelation is attributed to copper-free click reactions between an azide and dibenzyl cyclooctyne. HA-PEG4-DBCO was synthesized and cross-linked via 4-arm PEG azide. The effects of the ratio of HA-PEG4-DBCO to 4-arm PEG azide on the gelation time, microstructure, surface morphology, equilibrium swelling, and compressive modulus were examined. The potential of a hydrogel as an injectable scaffold was demonstrated by the encapsulation of chondrocytes within the hydrogel matrix in vitro and in vivo. The results demonstrated that the hydrogel supported cell survival, and the cells regenerated cartilaginous tissue. In addition, these characteristics provide potential opportunities for the use of injectable hydrogels in tissue engineering applications.

AB - We report a biocompatible and in situ cross-linkable hydrogel derived from hyaluronic acid via a bioorthogonal reaction and confirm the clinical potential of our hydrogel through in vivo cartilage regeneration. Gelation is attributed to copper-free click reactions between an azide and dibenzyl cyclooctyne. HA-PEG4-DBCO was synthesized and cross-linked via 4-arm PEG azide. The effects of the ratio of HA-PEG4-DBCO to 4-arm PEG azide on the gelation time, microstructure, surface morphology, equilibrium swelling, and compressive modulus were examined. The potential of a hydrogel as an injectable scaffold was demonstrated by the encapsulation of chondrocytes within the hydrogel matrix in vitro and in vivo. The results demonstrated that the hydrogel supported cell survival, and the cells regenerated cartilaginous tissue. In addition, these characteristics provide potential opportunities for the use of injectable hydrogels in tissue engineering applications.

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In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering

Abstract

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