In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering

Sang Soo Han, Hong Yeol Yoon, Ji Young Yhee, Myeong Ok Cho, Hye Eun Shim, Ji Eun Jeong, Dong Eun Lee, Kwang Meyung Kim, Hwanuk Guim, John Hwan Lee, Kang Moo Huh, Sun Woong Kang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We report a biocompatible and in situ cross-linkable hydrogel derived from hyaluronic acid via a bioorthogonal reaction and confirm the clinical potential of our hydrogel through in vivo cartilage regeneration. Gelation is attributed to copper-free click reactions between an azide and dibenzyl cyclooctyne. HA-PEG4-DBCO was synthesized and cross-linked via 4-arm PEG azide. The effects of the ratio of HA-PEG4-DBCO to 4-arm PEG azide on the gelation time, microstructure, surface morphology, equilibrium swelling, and compressive modulus were examined. The potential of a hydrogel as an injectable scaffold was demonstrated by the encapsulation of chondrocytes within the hydrogel matrix in vitro and in vivo. The results demonstrated that the hydrogel supported cell survival, and the cells regenerated cartilaginous tissue. In addition, these characteristics provide potential opportunities for the use of injectable hydrogels in tissue engineering applications.

Original languageEnglish
Pages (from-to)20-27
Number of pages8
JournalPolymer Chemistry
Volume9
Issue number1
DOIs
Publication statusPublished - 2018 Jan 7
Externally publishedYes

Fingerprint

Click Chemistry
Hyaluronic acid
Hydrogels
Hydrogel
Cartilage
Hyaluronic Acid
Tissue Engineering
Tissue engineering
Copper
Azides
Gelation
Polyethylene glycols
Injections
Chondrocytes
Encapsulation
Scaffolds
Surface morphology
Swelling
Regeneration
Cell Survival

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Polymers and Plastics
  • Organic Chemistry

Cite this

Han, S. S., Yoon, H. Y., Yhee, J. Y., Cho, M. O., Shim, H. E., Jeong, J. E., ... Kang, S. W. (2018). In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering. Polymer Chemistry, 9(1), 20-27. https://doi.org/10.1039/c7py01654a

In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering. / Han, Sang Soo; Yoon, Hong Yeol; Yhee, Ji Young; Cho, Myeong Ok; Shim, Hye Eun; Jeong, Ji Eun; Lee, Dong Eun; Kim, Kwang Meyung; Guim, Hwanuk; Lee, John Hwan; Huh, Kang Moo; Kang, Sun Woong.

In: Polymer Chemistry, Vol. 9, No. 1, 07.01.2018, p. 20-27.

Research output: Contribution to journalArticle

Han, SS, Yoon, HY, Yhee, JY, Cho, MO, Shim, HE, Jeong, JE, Lee, DE, Kim, KM, Guim, H, Lee, JH, Huh, KM & Kang, SW 2018, 'In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering', Polymer Chemistry, vol. 9, no. 1, pp. 20-27. https://doi.org/10.1039/c7py01654a
Han, Sang Soo ; Yoon, Hong Yeol ; Yhee, Ji Young ; Cho, Myeong Ok ; Shim, Hye Eun ; Jeong, Ji Eun ; Lee, Dong Eun ; Kim, Kwang Meyung ; Guim, Hwanuk ; Lee, John Hwan ; Huh, Kang Moo ; Kang, Sun Woong. / In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering. In: Polymer Chemistry. 2018 ; Vol. 9, No. 1. pp. 20-27.
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