In situ detection of live cancer cells by using bioprobes based on Au nanoparticles

Jaemoon Yang, Kilho Eom, Eun Kyung Lim, Jinsung Park, Yoonah Kang, Dae Sung Yoon, Sungsoo Na, Eui Kwan Koh, Jin Suck Suh, Yong Min Huh, Tae Yun Kwon, Seungjoo Haam

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We fabricate the high-performance probes based on Au nanoparticles (AuNP) for detection of live cancer cell. AuNP were synthesized with narrow sized distribution (ca. 10 nm) by Au salt reduction method and deposited onto the aminated substrate as a cross-linker and hot spot. Herein, AuNP has enabled the easy and efficient immobilization of the antibody (Cetuximab), which can selectively interact with epidermal growth factor receptor (EGFR) on the surface of epidermal cancer, as detecting moiety onto the AuNP-deposited substrate without nanolithography process. After conjugation of Cetuximab with AuNP-deposited substrate, Cetuximab-conjugated probe as a live cancer cell detector (LCCD) could detect EGFR-highexpressed A431 cells related to epithelial cancer with 54-times larger specificity and sensitivity in comparison with EGFR-deficient MCF7 cells. This implies that AuNP-based probes demonstrate abundant potentials for detection and separation of small biomolecules, cells and other chemicals.

Original languageEnglish
Pages (from-to)12112-12115
Number of pages4
JournalLangmuir
Volume24
Issue number21
DOIs
Publication statusPublished - 2008 Nov 4

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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    Yang, J., Eom, K., Lim, E. K., Park, J., Kang, Y., Yoon, D. S., Na, S., Koh, E. K., Suh, J. S., Huh, Y. M., Kwon, T. Y., & Haam, S. (2008). In situ detection of live cancer cells by using bioprobes based on Au nanoparticles. Langmuir, 24(21), 12112-12115. https://doi.org/10.1021/la802184m