In situ detoxification of lignocellulosic hydrolysate using a surfactant for butyric acid production by Clostridium tyrobutyricum ATCC 25755

Kyung Min Lee, Ki Yeon Kim, Okkyoung Choi, Han Min Woo, Yunje Kim, Sung Ok Han, Byoung In Sang, Youngsoon Um

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Lignocellulosic degradation compounds, especially phenolic compounds, inhibit the fermentation of Clostridium strains. In this study, a simple in situ detoxification method using a surfactant was developed for butyric acid production by Clostridium tyrobutyricum ATCC25755. Tween 80, a non-ionic surfactant, was chosen to sequester inhibitors by forming micelles, consequently preventing direct contact of inhibitors with cell membranes. When Tween 80 was added during fermentation, butyric acid production was significantly enhanced in the presence of hydrophobic phenolics such as p-coumaric acid (0 vs. 3.1 g/L butyric acid) and ferulic acid (2.2 vs. 4.6 g/L butyric acid) even at 0.016 g/L of Tween 80 (corresponding to the critical micelle concentration). Lignin, a polyphenol compound in lignocellulose, was also detoxified by Tween 80. When Tween 80 was added to acid-pretreated rice straw hydrolysate, butyric acid production significantly increased (0.1 vs. 8.7 g/L butyric acid), thus verifying detoxification effect of Tween 80 on lignocellulosic hydrolysate.

Original languageEnglish
Pages (from-to)630-635
Number of pages6
JournalProcess Biochemistry
Volume50
Issue number4
DOIs
Publication statusPublished - 2015

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Clostridium tyrobutyricum
Clostridium
Detoxification
Butyric acid
Butyric Acid
Polysorbates
Surface-Active Agents
Surface active agents
ferulic acid
Micelles
Fermentation
Acids
Critical micelle concentration
Lignin
Nonionic surfactants
Straw
Polyphenols
Cell membranes
Cell Membrane
Degradation

Keywords

  • Butyric acid
  • In situ detoxification
  • Lignocellulose
  • Phenolic compound
  • Tween 80

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

In situ detoxification of lignocellulosic hydrolysate using a surfactant for butyric acid production by Clostridium tyrobutyricum ATCC 25755. / Lee, Kyung Min; Kim, Ki Yeon; Choi, Okkyoung; Woo, Han Min; Kim, Yunje; Han, Sung Ok; Sang, Byoung In; Um, Youngsoon.

In: Process Biochemistry, Vol. 50, No. 4, 2015, p. 630-635.

Research output: Contribution to journalArticle

Lee, Kyung Min ; Kim, Ki Yeon ; Choi, Okkyoung ; Woo, Han Min ; Kim, Yunje ; Han, Sung Ok ; Sang, Byoung In ; Um, Youngsoon. / In situ detoxification of lignocellulosic hydrolysate using a surfactant for butyric acid production by Clostridium tyrobutyricum ATCC 25755. In: Process Biochemistry. 2015 ; Vol. 50, No. 4. pp. 630-635.
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