In situ imaging of quantum dot-AZD4547 conjugates for tracking the dynamic behavior of fibroblast growth factor receptor 3

Gyoyeon Hwang, Hyeonhye Kim, Hojong Yoon, Chiman Song, Dong-Kwon Lim, Taebo Sim, Jiyeon Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fibroblast growth factor receptors (FGFRs) play an important role in determining cell proliferation, differentiation, migration, and survival. Although a variety of small-molecule FGFR inhibitors have been developed for cancer therapeutics, the interaction between FGFRs and FGFR inhibitors has not been well characterized. The FGFR–inhibitor interaction can be characterized using a new imaging probe that has strong, stable signal properties for in situ cellular imaging of the interaction without quenching. We developed a kinase–inhibitor-modified quantum dot (QD) probe to investigate the interaction between FGFR and potential inhibitors. Especially, turbo-green fluorescent protein-FGFR3s were overexpressed in HeLa cells to investigate the colocalization of FGFR3 and AZD4547 using the QD-AZD4547 probe. The result indicates that this probe is useful for investigating the binding behaviors of FGFR3 with the FGFR inhibitor. Thus, this new inhibitor-modified QD probe is a promising tool for understanding the interaction between FGFR and inhibitors and for creating future high-content, cell-based drug screening strategies.

Original languageEnglish
Pages (from-to)5345-5357
Number of pages13
JournalInternational Journal of Nanomedicine
Volume12
DOIs
Publication statusPublished - 2017 Jul 26

Fingerprint

Receptor, Fibroblast Growth Factor, Type 3
Fibroblast Growth Factor Receptors
Quantum Dots
Fibroblasts
Semiconductor quantum dots
Imaging techniques
Growth Inhibitors
Preclinical Drug Evaluations
Cell proliferation
Green Fluorescent Proteins
AZD4547
Intercellular Signaling Peptides and Proteins
HeLa Cells
Cell Differentiation
Quenching
Screening
Cell Proliferation
Proteins
Molecules

Keywords

  • AZD4547
  • Fibroblast growth factor 3
  • In situ imaging
  • Inhibitor
  • Kinase
  • Quantum dot

ASJC Scopus subject areas

  • Bioengineering
  • Biophysics
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

In situ imaging of quantum dot-AZD4547 conjugates for tracking the dynamic behavior of fibroblast growth factor receptor 3. / Hwang, Gyoyeon; Kim, Hyeonhye; Yoon, Hojong; Song, Chiman; Lim, Dong-Kwon; Sim, Taebo; Lee, Jiyeon.

In: International Journal of Nanomedicine, Vol. 12, 26.07.2017, p. 5345-5357.

Research output: Contribution to journalArticle

Hwang, Gyoyeon ; Kim, Hyeonhye ; Yoon, Hojong ; Song, Chiman ; Lim, Dong-Kwon ; Sim, Taebo ; Lee, Jiyeon. / In situ imaging of quantum dot-AZD4547 conjugates for tracking the dynamic behavior of fibroblast growth factor receptor 3. In: International Journal of Nanomedicine. 2017 ; Vol. 12. pp. 5345-5357.
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