In situ real-time monitoring of biomolecular interactions based on resonating microcantilevers immersed in a viscous fluid

Tae Yun Kwon, Kilho Eom, Jae Hong Park, Dae Sung Yoon, Tae Song Kim, Hong Lim Lee

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The authors report the precise (noise-free) in situ real-time monitoring of a specific protein antigen-antibody interaction by using a resonating microcantilever immersed in a viscous fluid. In this work, they utilized a resonating piezoelectric thick film microcantilever, which exhibits the high quality factor (e.g., Q=15) in a viscous liquid at a viscosity comparable to that of human blood serum. This implies a great potential of the resonating microcantilever to in situ biosensor applications. It is shown that the microcantilever enables them to monitor the C reactive protein antigen-antibody interactions in real time, providing an insight into the protein binding kinetics.

Original languageEnglish
Article number223903
JournalApplied Physics Letters
Volume90
Issue number22
DOIs
Publication statusPublished - 2007 Jun 11
Externally publishedYes

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viscous fluids
antigens
proteins
antibodies
blood serum
interactions
bioinstrumentation
thick films
Q factors
viscosity
kinetics
liquids

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

In situ real-time monitoring of biomolecular interactions based on resonating microcantilevers immersed in a viscous fluid. / Kwon, Tae Yun; Eom, Kilho; Park, Jae Hong; Yoon, Dae Sung; Kim, Tae Song; Lee, Hong Lim.

In: Applied Physics Letters, Vol. 90, No. 22, 223903, 11.06.2007.

Research output: Contribution to journalArticle

Kwon, Tae Yun ; Eom, Kilho ; Park, Jae Hong ; Yoon, Dae Sung ; Kim, Tae Song ; Lee, Hong Lim. / In situ real-time monitoring of biomolecular interactions based on resonating microcantilevers immersed in a viscous fluid. In: Applied Physics Letters. 2007 ; Vol. 90, No. 22.
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